Aruna Adimoolam, Wang Tsan-Ping, Cao Jyun-Cing, Lan Dan-Suei, Nagarajan Ganesan, Chang Ching-Fong
Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan.
Department of Basic Sciences, PYD, King Faisal University, Al Hofuf, Saudi Arabia.
Front Physiol. 2021 Nov 11;12:768122. doi: 10.3389/fphys.2021.768122. eCollection 2021.
The local gill production of corticotropin releasing hormone () and -receptor () is hypothesized to play important roles during seawater (SW) and freshwater (FW) acclimation in euryhaline black porgy (). The mRNA expression of , , and / () was examined in SW and FW diencephalon (Dien) and in the gills at different exposure time by Q-PCR analysis. The hybridization results indicate that mRNA hybridization signals were more abundant in FW fish in the gigantocellular (PMgc) and parvocellular (PMpc) part of the magnocellular preoptic nucleus versus SW fish. The and expressing cells were located in basal cells of gill filament. Furthermore, dexamethasone (DEX) treatment could increase the -system in the gill. Increased transcripts of the -system in the gill via and CRH treatments suggest that CRH may regulate the system in a local manner. The a-Nka cells were localized in the filament and secondary lamellae mitochondria rich cells (MRCs) of FW fish at 8 h and 1 day. a-Nka cells were seen in both filament and lamellae in the FW but much less in SW fish indicating that gills play key roles in black porgy osmoregulation. Gill and play important roles in the response to salinity stress.
据推测，广盐性黑鲷在适应海水（SW）和淡水（FW）环境过程中，其鳃局部促肾上腺皮质激素释放激素（）和 - 受体（）的产生发挥着重要作用。通过实时定量聚合酶链反应（Q-PCR）分析，检测了在不同暴露时间下，SW和FW环境中黑鲷间脑（Dien）以及鳃中、和 / （）的mRNA表达情况。杂交结果表明，与SW环境中的黑鲷相比，FW环境中的黑鲷在大细胞视前核的巨细胞部（PMgc）和小细胞部（PMpc）中，mRNA杂交信号更为丰富。和表达细胞位于鳃丝的基底细胞中。此外，地塞米松（DEX）处理可增加鳃中的 - 系统。通过和促肾上腺皮质激素释放激素（CRH）处理增加鳃中 - 系统的转录本，表明CRH可能以局部方式调节该系统。在8小时和1天时，a - Nka细胞定位于FW环境中黑鲷的鳃丝和次生薄片富含线粒体的细胞（MRCs）中。在FW环境中的黑鲷的鳃丝和薄片中均可见a - Nka细胞，但在SW环境中的黑鲷中则少得多，这表明鳃在黑鲷的渗透压调节中起关键作用。鳃中的和在应对盐度胁迫中发挥重要作用。
