Department of Basic Sciences, PYD, King Faisal University, Al Ahsa 31982, Saudi Arabia.
Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung 20224, Taiwan.
Int J Mol Sci. 2023 Mar 10;24(6):5318. doi: 10.3390/ijms24065318.
In euryhaline teleost black porgy, , the glucocorticoid receptor (), growth hormone receptor (), prolactin ()-receptor (), and sodium-potassium ATPase alpha subunit () play essential physiological roles in the osmoregulatory organs, including the gill, kidney, and intestine, during osmotic stress. The present study aimed to investigate the impact of pituitary hormones and hormone receptors in the osmoregulatory organs during the transfer from freshwater (FW) to 4 ppt and seawater (SW) and vice versa in black porgy. Quantitative real-time PCR (Q-PCR) was carried out to analyze the transcript levels during salinity and osmoregulatory stress. Increased salinity resulted in decreased transcripts of in the pituitary, and in the gill, and and in the kidney. Increased salinity caused the increased transcripts of in the gill and in the intestine. Decreased salinity resulted in increased pituitary , and increases in and in the gill, and , , and in the kidney. Taken together, the present results highlight the involvement of , , , and in the osmoregulation and osmotic stress in the osmoregulatory organs (gill, intestine, and kidney). Pituitary , and gill and intestine are consistently downregulated during the increased salinity stress and vice versa. It is suggested that plays a more significant role in osmoregulation than in the euryhaline black porgy. Furthermore, the present results highlighted that the gill transcript's role was solely to balance the homeostasis in the black porgy during salinity stress.
在广盐性硬骨鱼黑鲷中，糖皮质激素受体（）、生长激素受体（）、催乳素（）-受体（）和钠钾-ATP 酶α亚基（）在渗透压应激时，在包括鳃、肾脏和肠道在内的渗透压调节器官中发挥重要的生理作用。本研究旨在研究在黑鲷从淡水（FW）转移到 4 ppt 和海水（SW）以及反之亦然的过程中，脑垂体激素和激素受体对渗透压调节器官的影响。采用定量实时 PCR（Q-PCR）分析盐度和渗透压应激过程中调节器官中的转录水平。盐度升高导致脑垂体中的 、鳃中的 和肾脏中的 转录物减少。盐度升高导致鳃中的 转录物增加和肠道中的 增加。盐度降低导致脑垂体中的 增加，以及鳃和肾脏中的 、 、和 增加。总之，本研究结果表明 、 、 、和 参与了渗透压调节器官（鳃、肠和肾）中的渗透压调节和渗透胁迫。在增加的盐度胁迫期间，脑垂体 、和鳃和肠道 持续下调，反之亦然。这表明在广盐性黑鲷中， 比 在渗透压调节中发挥更重要的作用。此外，本研究结果强调了在盐度应激期间，鳃 转录物的作用仅是为了平衡黑鲷的体内平衡。
