Department of Aquaculture, National Taiwan Ocean University, Keelung 20224, Taiwan.
Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung 91230, Taiwan.
Cells. 2021 Nov 9;10(11):3086. doi: 10.3390/cells10113086.
Our study showed differential expression of the ()/ () in the brain and pituitary gland of the euryhaline black porgy () during osmotic stress. A decrease in serum osmolality and increased cortisol levels were observed after acute transfer from seawater (SW) to freshwater (FW). The increased expressions of , (: ), and (: transcripts on day 1 and and transcripts on days 7 and 30 were found in the brains and pituitary glands of FW fish. Increased levels of mRNA in the diencephalon and mRNA in the pituitary together with serum cortisol on day 1 of FW exposure indicated activation of the hypothalamic-pituitary-interrenal (HPI) axis. The expression levels of and after FW transfer were increased in the pituitary on days 7 and 30. Furthermore, hybridization demonstrated spatially differential expression of and transcripts in nucleus preopticus parvocellularis of pars gigantocellularis (PMgc), magnocellularis (PMmc), and parvocellularis (PMpc) of the preoptic area (POA). Positive signals for and were highly abundant in PMpc after FW exposure. The data suggest involvement of neurohypophysial hormones in the brain (telencephalon and diencephalon) and pituitary for osmotic stress.
我们的研究表明，在渗透胁迫下，广盐性黑鲷的脑和垂体中（）/（）的表达存在差异。从海水（SW）急性转移到淡水（FW）后，观察到血清渗透压降低和皮质醇水平升高。在 FW 鱼的脑和垂体中，发现（:）、（:）和（:）转录本在第 1 天，和转录本在第 7 天和第 30 天的表达增加。FW 暴露第 1 天，间脑的 mRNA 水平和垂体的 mRNA 水平以及血清皮质醇水平升高，表明下丘脑-垂体-肾上腺（HPI）轴被激活。FW 转移后，垂体中 和 的表达水平在第 7 天和第 30 天增加。此外，原位杂交显示，在视前区（POA）的大细胞部（PMmc）、巨细胞部（PMgc）和小细胞部（PMpc）的前脑下垂体核（PMgc）中，和转录本的表达存在空间差异。FW 暴露后，PMpc 中 和 的阳性信号高度丰富。这些数据表明神经垂体激素参与了脑（端脑和间脑）和垂体的渗透压应激反应。
