Spits H, van Schooten W, Keizer H, van Seventer G, van de Rijn M, Terhorst C, de Vries J E
Science. 1986 Apr 18;232(4748):403-5. doi: 10.1126/science.3485822.
T-cell receptors bind antigens only when the antigens are exposed on the cell surface. This can be studied best in the interaction of cytolytic T lymphocytes (CTL) with target cells because the recognition and binding event can be separated from the lytic phase. Studies with CTL clones specific for HLA-A2 and HLA-B7 demonstrated that conjugates of CTL's and target cells can be formed in the absence of specific antigen recognition. Furthermore, T-cell receptor and target antigen cannot interact unless there is conjugate formation. This indicates that nonspecific conjugate formation between CTL's and target cells precedes the recognition of specific antigen by the T-cell receptor.
只有当抗原暴露在细胞表面时，T细胞受体才会与之结合。这一点在细胞毒性T淋巴细胞（CTL）与靶细胞的相互作用中研究得最为透彻，因为识别和结合事件可以与裂解阶段分开。对针对HLA - A2和HLA - B7的CTL克隆的研究表明，在没有特异性抗原识别的情况下，CTL与靶细胞也能形成结合物。此外，除非形成结合物，T细胞受体和靶抗原无法相互作用。这表明CTL与靶细胞之间非特异性结合物的形成先于T细胞受体对特异性抗原的识别。
