O'Connor Sean P, Powell Samantha M, Rickman John M, Pope Nathaniel J, Noojin Gary D, Scully Marlan O, Denton Michael L, Yakovlev Vladislav V
Department of Physics and Astronomy, Texas A&M University, College Station, TX 77843, USA.
National Research Council Postdoctoral Fellow, JBSA Fort Sam Houston, TX 78234, USA.
Biomed Opt Express. 2021 Oct 22;12(11):7082-7091. doi: 10.1364/BOE.441118. eCollection 2021 Nov 1.
The electronic or molecular mechanisms that initiate photobiomodulation (PBM) in cells are not yet fully understood. The porcine complex III (C-III) of the electron transport chain was characterized with transient absorption spectroscopy (TAS). We then applied our recently developed continuous wave laser coupled TAS procedure (CW-TAS) to investigate the effect of red light irradiances on the heme dynamics of C-III in its c reduced state. The time constants were found to be 3.3 ± 0.3 ps for vibrational cooling of the oxidized state and 4.9 ± 0.4 ps for rebinding of the photodissociated axial ligand of the c reduced state. The analysis of the CW-TAS procedure yielded no significant changes in the C-III heme dynamics. We rule out the possibility of 635 nm CW light at 4.7 mW/cm inducing a PBM effect on the heme dynamic of C-III, specifically with the photodissociation of its axial ligand.
细胞中引发光生物调节（PBM）的电子或分子机制尚未完全明确。利用瞬态吸收光谱（TAS）对电子传递链的猪复合物III（C-III）进行了表征。随后，我们应用最近开发的连续波激光耦合TAS程序（CW-TAS）来研究红光辐照度对处于c还原态的C-III血红素动力学的影响。发现氧化态振动冷却的时间常数为3.3±0.3皮秒，c还原态光解离轴向配体重新结合的时间常数为4.9±0.4皮秒。CW-TAS程序分析结果表明C-III血红素动力学没有显著变化。我们排除了4.7 mW/cm的635 nm连续波光对C-III血红素动力学产生PBM效应的可能性，特别是其轴向配体的光解离。
