Power D A, Mather A J, MacLeod A M, Lind T, Catto G R
Am J Reprod Immunol Microbiol. 1986 Jan;10(1):10-3. doi: 10.1111/j.1600-0897.1986.tb00002.x.
Sera were obtained prior to conception and during the first trimester of subsequent pregnancies from 22 women over 27 pregnancies; on 15 occasions these pregnancies ended in spontaneous abortion, whereas the remaining 12 achieved live normal babies. In only one pregnancy ending in abortion could antibodies directed to paternal B-lymphocytes determined by the EA rosette inhibition (EAI) assay be detected in the mother's serum, whereas five of the 12 successful pregnancies were associated with such detectable antibodies. Cytotoxic antibodies were also found in all but one of these EAI-positive pregnancies and no antibody activity was present in sera obtained from five primigravidae. These results indicated that normal, but not abnormal, pregnancies were often associated with blocking antibody formation, suggesting that such antibodies may protect the fetus from abortion. However, the failure to detect antibody activity in sera from first trimester primigravidae argues against a central role for blocking antibody, alone, in the maintenance of outbred pregnancy.
在22名女性的27次以上后续妊娠中，于受孕前及妊娠头三个月采集血清。其中15次妊娠以自然流产告终，其余12次成功产下正常活婴。在仅有的一次流产妊娠中，通过EA花环抑制（EAI）试验测定，在母亲血清中可检测到针对父方B淋巴细胞的抗体，而在12次成功妊娠中有5次与可检测到此类抗体有关。在这些EAI阳性妊娠中，除一例之外，其余均发现有细胞毒性抗体，且从5名初产妇获得的血清中未检测到抗体活性。这些结果表明，正常而非异常妊娠通常与封闭抗体的形成有关，提示此类抗体可能保护胎儿免于流产。然而，在妊娠头三个月初产妇的血清中未检测到抗体活性，这表明封闭抗体单独在维持远交妊娠方面不发挥核心作用。
