Innes A, Cunningham C, Power D A, Catto G R
Department of Medicine and Therapeutics, University of Aberdeen, Scotland.
Am J Reprod Immunol. 1990 Jun;23(2):50-3. doi: 10.1111/j.1600-0897.1990.tb00670.x.
In this study we have compared the ability of two assay systems, erythrocyte antibody rosette inhibition (EAI) and cellular enzyme-linked immunospecific assay (CELISA) to detect maternal alloantibody activity during pregnancy. Antibody activity to antigens on paternal lymphocytes was present in nine of 23 primigravid sera tested by EAI and in seven of 23 by CELISA. In multiparous sera, antibodies directed to paternal lymphocytes were detected in 11 of 15 individuals by EAI and in six of 15 by CELISA. The techniques correlated significantly when assaying the humoral response in sera from multiparous women. The lack of correlation when assaying primigravid sera suggests that both assays encounter difficulty in detecting the low titres of antibodies present.
在本研究中，我们比较了两种检测系统，即红细胞抗体玫瑰花结抑制试验（EAI）和细胞酶联免疫特异性试验（CELISA）在孕期检测母体同种抗体活性的能力。通过EAI检测的23份初孕妇血清中，有9份对父源淋巴细胞上的抗原有抗体活性；通过CELISA检测的23份血清中，有7份有抗体活性。在经产妇血清中，通过EAI在15人中检测到11人有针对父源淋巴细胞的抗体，通过CELISA在15人中检测到6人有抗体。在检测经产妇血清中的体液反应时，这两种技术有显著相关性。检测初孕妇血清时缺乏相关性，这表明两种检测方法在检测低滴度抗体时都存在困难。
