International Medical University, Department of Pathology, Kuala Lumpur, Malaysia.
Med J Malaysia. 2023 Mar;78(2):202-206.
The purpose of tissue processing is to fix the tissue in a solid medium toenable thin sections. Conventional method of tissue processing is the standardized method of tissue processing which has been used for more than 10 decades. However, the conventional method is time-consuming, and the overall turnaround time for the histopathology report is at least two days. The objective of this study is to identify the protocol for tissue processing procedure using domestic microwave oven. To determine the tissue processing time when using domestic microwave oven. To compare the morphological quality of tissue slides made by domestic microwave oven and conventional method using automated tissue processor.
The conventional protocol and three microwave protocols of tissue processing were used in this study. A pilot study was done prior to the real run to determine the baseline timing for microwave protocol. The baseline timing was fixed at 2 minutes,30 minutes,5 minutes and 25 minutes. The processing time of the microwave protocol was adjusted from 62 minutes to 70 minutes to 77 minutes by increasing the dehydration and wax impregnation time while the time for tissue fixation and clearing remain the same throughout all the microwave protocols.
The group 2 microwave protocol produced the sections that is closely comparable to group 1 conventional protocol. The morphological quality of histopathology slides is best observed when the processing time of microwave protocol is 62 minutes.
The most appropriate microwave protocol for tissue processing is group 2 as the morphological quality of histopathology slides are more superior than that of group 1 with an overall percentage of 80% of satisfactory slides in group 2 and 76.68% in group 1.
组织处理的目的是将组织固定在固体介质中，以便制作薄片。传统的组织处理方法是标准化的组织处理方法，已经使用了超过 10 个十年。然而，传统方法耗时较长，组织病理学报告的总体周转时间至少为两天。本研究的目的是确定使用国产微波炉进行组织处理程序的方案。确定使用国产微波炉进行组织处理的时间。比较国产微波炉和常规方法使用自动化组织处理器制作的组织切片的形态质量。
本研究使用了传统方案和三种微波组织处理方案。在实际运行之前进行了一项试点研究，以确定微波方案的基线时间。基线时间固定为 2 分钟、30 分钟、5 分钟和 25 分钟。通过增加脱水和浸蜡时间来调整微波方案的处理时间，从 62 分钟调整到 70 分钟再到 77 分钟，而所有微波方案中的组织固定和透明时间保持不变。
第 2 组微波方案产生的切片与第 1 组常规方案非常相似。当微波方案的处理时间为 62 分钟时，观察组织病理学切片的形态质量最佳。
最适合组织处理的微波方案是第 2 组，因为第 2 组组织病理学切片的形态质量优于第 1 组，第 2 组中 80%的切片令人满意，第 1 组中为 76.68%。
