The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Fujian Provincial Key Laboratory of Chemical Biology, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
MOE Key Laboratory for Analytical Science of Food Safety and Biology, College of Chemistry, Fuzhou University, Fujian Science & Technology Innovation Laboratory for Optoelectronic Information of China, Fuzhou 350108, China.
Anal Chem. 2021 Dec 14;93(49):16552-16561. doi: 10.1021/acs.analchem.1c03744. Epub 2021 Dec 3.
levels of reactive oxygen species (ROS) and reactive nitrogen species (RNS) are critical to many physiological and pathological processes. Because of the distinct differences in their biological generation and effects, simultaneously visualizing both of them could help deepen our insights into the mechanistic details of these processes. However, real-time and deep-tissue imaging and differentiation of ROS- and RNS-related molecular events in living subjects still remain a challenge. Here, we report the development of two activatable F magnetic resonance imaging (MRI) molecular probes with different F chemical shifts and specific responsive behaviors for simultaneous detection and deep-tissue imaging of O and ONOO. These probes are capable of real-time visualization and differentiation of O and ONOO in living mice with drug-induced acute kidney injury by interference-free multiplexed hot-spot F MRI, illustrating the potential of this technique for background-free real-time imaging of diverse biological processes, accurate diagnosis of various diseases in deep tissues, and rapid toxicity evaluation of assorted drugs.
活性氧（ROS）和活性氮（RNS）的水平对许多生理和病理过程至关重要。由于它们在生物生成和作用方面存在明显差异，因此同时对两者进行可视化可以帮助我们更深入地了解这些过程的机制细节。然而，在活体中实时、深层组织成像并区分与 ROS 和 RNS 相关的分子事件仍然是一个挑战。在这里，我们报告了两种具有不同 F 化学位移和特定响应行为的可激活 F 磁共振成像（MRI）分子探针的开发，用于同时检测和深层组织成像 O 和 ONOO。这些探针能够通过无干扰的多重热点 F MRI 实时可视化和区分活体小鼠药物诱导的急性肾损伤中的 O 和 ONOO，这表明该技术具有用于无背景实时成像多种生物过程、准确诊断深层组织中各种疾病以及快速评估各种药物毒性的潜力。
