Lin Hongyu, Tang Xiaoxue, Li Ao, Gao Jinhao
State Key Laboratory of Physical Chemistry of Solid Surfaces, The MOE Laboratory of Spectrochemical Analysis & Instrumentation, The Key Laboratory for Chemical Biology of Fujian Province, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
Adv Mater. 2021 Dec;33(50):e2005657. doi: 10.1002/adma.202005657. Epub 2021 Apr 8.
Visualization of biological targets such as crucial cells and biomolecules in living subjects is critical for the studies of important biological processes. Though H magnetic resonance imaging (MRI) has demonstrated its power in offering detailed anatomical and pathological information, its capacity for in vivo tracking of biological targets is limited by the high biological background of H. F distinguishes itself from its competitors as an exceptional complement to H in MRI through its high sensitivity, low biological background, and broad chemical shift range. The specificity and sensitivity of F MRI can be further boosted with activatable nanoprobes. The advantages of F MRI with activatable nanoprobes enable in vivo detection and imaging at the cellular or even molecular level in deep tissues, rendering this technique appealing as a potential solution for visualization of biological targets in living subjects. Here, recent progress over the past decades on activatable F MRI nanoprobes made from three major F-containing compounds, as well as present challenges and potential opportunities, are summarized to provide a panoramic prospective for the people who are interested in this emerging and exciting field.
对活体受试者体内关键细胞和生物分子等生物靶点进行可视化，对于重要生物过程的研究至关重要。尽管氢磁共振成像（MRI）已展现出其在提供详细解剖和病理信息方面的能力，但其对生物靶点进行体内追踪的能力受到氢高生物背景的限制。氟在MRI中作为氢的特殊补充脱颖而出，因其具有高灵敏度、低生物背景和宽化学位移范围。可激活纳米探针能够进一步提高氟MRI的特异性和灵敏度。含氟可激活纳米探针的氟MRI优势使其能够在深部组织的细胞甚至分子水平进行体内检测和成像，使该技术成为活体受试者生物靶点可视化的潜在解决方案，具有吸引力。在此，总结了过去几十年中由三种主要含氟化合物制成的可激活氟MRI纳米探针的最新进展，以及当前面临的挑战和潜在机遇，为对这一新兴且令人兴奋的领域感兴趣的人们提供全景展望。
