Department of Mining and Materials Engineering, McGill University, Montreal, Quebec H3A 2T5, Canada.
Faculty of Dentistry, McGill University, Montreal, Quebec H3A 2T5, Canada.
Langmuir. 2021 Dec 14;37(49):14243-14253. doi: 10.1021/acs.langmuir.1c01593. Epub 2021 Dec 3.
Bioactive glasses are surface-reactive glasses that, when placed in physiological fluid, undergo a transformation from glass to hydroxyapatite. Doping the bioactive glass with metallic ions can impart desirable and unique properties that are not inherent to natural hydroxyapatite. Once such ion is titanium. Titanium exists in trace amounts in native dental enamel, and its presence has been correlated with increased tooth hardness and brightness, both desirable clinical properties. Synthetic titanium-substituted hydroxyapatite exhibits better mechanical and antibacterial properties and demonstrates potential for an improved cellular response when compared to unmodified hydroxyapatite with applications in the broader field of bone tissue engineering. In this work, we use the sol-gel method to synthesize a titanium-containing silicate-based bioactive glass aimed at generating titanium-substituted hydroxyapatite on the glass surface upon immersion in body fluid. Titanium is homogeneously distributed throughout our glass, which keeps its amorphous nature. After 14 days of immersion in simulated body fluid, the glass forms a titanium-substituted hydroxyapatite on its surface. Enamel surfaces treated with the titanium-containing glass show significantly increased microhardness compared to enamel surfaces treated with a control glass, confirming the potential for the proposed glass in enamel remineralization. We also show that the presence of titanium in the glass promotes cell differentiation toward bone formation, suggesting further applications for this material in the broader field of bone tissue engineering.
生物活性玻璃是一种表面反应性玻璃，当放置在生理流体中时，它会从玻璃转变为羟基磷灰石。在生物活性玻璃中掺杂金属离子可以赋予其天然羟基磷灰石所不具有的理想和独特的性质。其中一种离子是钛。钛在天然牙釉质中微量存在，其存在与牙齿硬度和亮度的增加有关，这都是理想的临床特性。与未改性的羟基磷灰石相比，合成的钛取代羟基磷灰石具有更好的机械性能和抗菌性能，并显示出在更广泛的骨组织工程领域中具有改善细胞反应的潜力。在这项工作中，我们使用溶胶-凝胶法合成了一种含钛的硅酸盐生物活性玻璃，旨在在浸入体液时在玻璃表面生成钛取代的羟基磷灰石。钛均匀分布在整个玻璃中，保持其非晶态。在模拟体液中浸泡 14 天后，玻璃在其表面形成了钛取代的羟基磷灰石。用含钛玻璃处理的牙釉质表面的显微硬度明显高于用对照玻璃处理的牙釉质表面，证实了所提出的玻璃在牙釉质再矿化中的潜力。我们还表明，玻璃中钛的存在促进了向骨形成的细胞分化，这表明该材料在更广泛的骨组织工程领域中有进一步的应用。
