Gutierrez C, Howard M, Gaspar M L, Raveche E S
Clin Immunol Immunopathol. 1986 May;39(2):319-28. doi: 10.1016/0090-1229(86)90095-4.
Highly purified B cells from NZB mice have altered responses to various stimuli which require additional costimulatory signals supplied by factors present in EL-4 supernatants when compared to age-matched control nonautoimmune strains. Both crude preparations of EL-4 supernatants as well as partially purified BSF-p1 induced peak proliferation in B cells from normal strains of mice only in the presence of another stimulatory signal, anti-mu. In contrast, B cells from autoimmune-prone NZB mice proliferated in response to B-cell growth factors, with an age-dependent variation. Splenic B cells from 16- to 22-week-old NZB mice, an age where pronounced autoimmune disease is not observed, demonstrated a near maximum proliferative response with B-cell growth factors alone. While normal B cells responded maximally to BSF-p1 in the presence of anti-mu, B cells from young adult NZB mice (16-22 weeks of age) were not further stimulated to proliferate upon the addition of anti-mu. Such NZB B cells appeared to lack the requirement for a stimulation signal delivered by anti-mu in order to respond to B-cell growth factors. These results suggested that NZB cells were partially activated in vivo in the preautoimmune state so that subliminal triggers lead to full activation.
与年龄匹配的对照非自身免疫品系相比，来自NZB小鼠的高度纯化B细胞对各种刺激的反应有所改变，这些刺激需要EL-4上清液中存在的因子提供额外的共刺激信号。EL-4上清液的粗制品以及部分纯化的BSF-p1仅在存在另一种刺激信号抗μ的情况下，才能诱导正常小鼠品系B细胞的峰值增殖。相比之下，易患自身免疫的NZB小鼠的B细胞会对B细胞生长因子作出反应，并呈现年龄依赖性变化。来自16至22周龄NZB小鼠的脾脏B细胞（这个年龄段未观察到明显的自身免疫疾病），仅在B细胞生长因子作用下就表现出近乎最大的增殖反应。虽然正常B细胞在抗μ存在时对BSF-p1反应最大，但来自年轻成年NZB小鼠（16至22周龄）的B细胞在添加抗μ后并未进一步被刺激增殖。此类NZB B细胞似乎在响应B细胞生长因子时，无需抗μ传递的刺激信号。这些结果表明，NZB细胞在自身免疫前状态下在体内被部分激活，因此阈下触发会导致完全激活。
