Voorter Pieter-Jan, McKay Alasdair, Dai Jinhuo, Paravagna Olga, Cameron Neil R, Junkers Tanja
Polymer Reaction Design Group, School of Chemistry, Monash University, 19 Rainforest Walk, Building 23, Clayton, VIC 3800, Australia.
Dulux Australia, 1956 Dandenong Road, Clayton, VIC 3168, Australia.
Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202114536. doi: 10.1002/anie.202114536. Epub 2021 Dec 14.
Diffusion-ordered NMR spectroscopy (DOSY) allows for accurate molecular weight calibration and determination that can be corrected for solvent influences. Polystyrene and poly(ethylene glycol) standards have been used to calibrate DOSY diffusion data for a variety of solvents, showing a high correlation of data when the bulk viscosity of the solvent is accounted for following the Stokes-Einstein equation. In this way, a type of universal calibration is introduced that allows for determinations of average molecular weight that are at least as accurate as those of traditional size-exclusion chromatography (SEC), if not better. Further, we demonstrate that DOSY calibrations can be used between laboratories, hence removing the need for individual calibration of setups as currently done.
扩散排序核磁共振光谱法（DOSY）可实现准确的分子量校准和测定，且能校正溶剂影响。聚苯乙烯和聚乙二醇标准品已用于校准多种溶剂的DOSY扩散数据，当根据斯托克斯-爱因斯坦方程考虑溶剂的本体粘度时，数据显示出高度相关性。通过这种方式，引入了一种通用校准类型，其可实现平均分子量的测定，即便不比传统尺寸排阻色谱法（SEC）更准确，至少也一样准确。此外，我们证明了DOSY校准可在不同实验室之间使用，因此无需像目前这样对各个装置进行单独校准。
