Bioengineering and Environmental Sciences Lab, Department of Energy and Environmental Engineering (DEE), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India.
Bioengineering and Environmental Sciences Lab, Department of Energy and Environmental Engineering (DEE), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India.
Bioresour Technol. 2022 Feb;345:126446. doi: 10.1016/j.biortech.2021.126446. Epub 2021 Nov 30.
Tetradesmus sp. SVMIICT4 was isolated and cultivated mixotrophically in a flat-panel photobioreactor (FP-PBR) for concurrent dairy wastewater treatment, carbon fixation, and biomass production. Integrated wastewater treatment showed good COD and nutrients removal efficiency accounting for biomass with an accumulation of carbohydrate (21.48 mg g) and protein (19.52 mg g). Chlorophyll a fluorescence transients (Fv/Fm, ETo/RC, TRo/RC, and Abs/RC) deduced through OJIP curve fittings, showed consistent improvement in photosynthetic activity throughout the cultivation period. The absorption flux per reaction centre corroborated with increased chlorophyll content (18.94 mg g), resulting in higher electron transport (ET/Rc) and lower non-photochemical quenching (NPQ). The fatty acid profile showed high content of unsaturated, followed by saturated fatty acids, which has multiple applications in food, feed, and fuel industries, enabling a bio-based economy.
从混合营养型平板光生物反应器(FP-PBR)中分离和培养 Tetradesmus sp. SVMIICT4,用于同时处理奶牛废水、固定碳和生产生物质。集成废水处理表现出良好的 COD 和营养物去除效率,占生物质的积累,包括碳水化合物(21.48mg/g)和蛋白质(19.52mg/g)。通过 OJIP 曲线拟合得出的叶绿素 a 荧光瞬变(Fv/Fm、ETo/RC、TRo/RC 和 Abs/RC)表明,在整个培养期间光合作用活性持续得到改善。每个反应中心的吸收通量与增加的叶绿素含量(18.94mg/g)相符,导致更高的电子传递(ET/Rc)和更低的非光化学猝灭(NPQ)。脂肪酸图谱显示出高含量的不饱和脂肪酸,其次是饱和脂肪酸,这在食品、饲料和燃料工业中有多种应用,使生物经济成为可能。