Departamento de Posgrado, Doctorado en Ciencias de la Ingeniería, Tecnológico Nacional de México en Celaya, Antonio García Cubas #600 Pte., Colonia Alfredo V. Bonfil, Apartado Postal 57, Celaya 38010, Guanajuato, Mexico.
Programa de Biotecnología, Universidad de Guanajuato, Mutualismo #303, Colonia La Suiza, Celaya 38060, Guanajuato, Mexico.
Mar Drugs. 2023 May 26;21(6):321. doi: 10.3390/md21060321.
The biomass of microalgae and the compounds that can be obtained from their processing are of great interest for various economic sectors. Chlorophyll from green microalgae has biotechnological applications of great potential in different industrial areas such as food, animal feed, pharmaceuticals, cosmetics, and agriculture. In this paper, the experimental, technical and economic performance of biomass production from a microalgal consortium ( sp., sp., sp., sp., sp., and sp.) was investigated in three cultivation systems (phototrophic, heterotrophic and mixotrophic) in combination with the extraction of chlorophyll ( and ) on a large scale using simulation; 1 ha was established as the area for cultivation. In the laboratory-scale experimental stage, biomass and chlorophyll concentrations were determined for 12 days. In the simulation stage, two retention times in the photobioreactor were considered, which generated six case studies for the culture stage. Subsequently, a simulation proposal for the chlorophyll extraction process was evaluated. The highest microalgae biomass concentration was 2.06 g/L in heterotrophic culture, followed by mixotrophic (1.98 g/L). Phototrophic and mixotrophic cultures showed the highest chlorophyll concentrations of 20.5 µg/mL and 13.5 µg/mL, respectively. The simulation shows that higher biomass and chlorophyll production is attained when using the mixotrophic culture with 72 h of retention that we considered to evaluate chlorophyll production ( and ). The operating cost of the entire process is very high; the cultivation stage has the highest operating cost (78%), mainly due to the high energy consumption of the photobioreactors.
微藻的生物量及其加工产物对许多经济领域都具有重要意义。来自绿藻的叶绿素在食品、动物饲料、制药、化妆品和农业等不同工业领域具有巨大的潜在生物技术应用价值。在本文中，我们研究了一种微藻联合体（ sp.、 sp.、 sp.、 sp.、 sp. 和 sp.）在三种培养系统（光合、异养和混合营养）中的生物量生产的实验、技术和经济性能，并结合大规模使用模拟法提取叶绿素（和）。假设 1 公顷为培养面积。在实验室规模的实验阶段，我们确定了 12 天内的生物量和叶绿素浓度。在模拟阶段，我们考虑了光生物反应器中的两个保留时间，这为培养阶段生成了六个案例研究。随后，我们评估了叶绿素提取工艺的模拟方案。在异养培养中，微藻生物量浓度最高为 2.06 g/L，其次是混合营养培养（1.98 g/L）。光合和混合营养培养的叶绿素浓度最高，分别为 20.5 µg/mL 和 13.5 µg/mL。模拟结果表明，当使用 72 小时保留时间的混合营养培养时，可以获得更高的生物量和叶绿素产量，我们认为这可以用来评估叶绿素的产量（和）。整个过程的运营成本非常高；培养阶段的运营成本最高（78%），主要是由于光生物反应器的高能耗。
