Department of Orthodontics, Medical Center of Stomatology, The Second Xiangya Hospital, Central South University, Changsha, 410011, Hunan, PR China.
Department of Stomatology, Affiliated Xiaoshan Hospital, Hangzhou Normal University, Hangzhou, 311202, Zhejiang, PR China.
J Mater Sci Mater Med. 2021 Dec 4;32(12):145. doi: 10.1007/s10856-021-06621-8.
Periodontitis is a progressive infectious inflammatory disease, which leads to alveolar bone resorption and loss of periodontal attachment. It is imperative for us to develop a therapeutic scaffold to repair the alveolar bone defect of periodontitis. In this study, we designed a new composite scaffold loading metformin (MET) by using the freeze-drying method, which was composed of β-tricalcium phosphate (β-TCP), chitosan (CTS) and the mesoporous silica (SBA-15). The scaffolds were expected to combine the excellent biocompatibility of CTS, the good bioactivity of β-TCP, and the anti-inflammatory properties of MET. The MET-loaded β-TCP/CTS/SBA-15 scaffolds showed improved cell adhesion, appropriate porosity and good biocompatibility in vitro. This MET composite scaffold was implanted in the alveolar bone defects area of rats with periodontitis. After 12 weeks, Micro-CT and histological analysis were performed to evaluate different degrees of healing and mineralization. Results showed that the MET-loaded β-TCP/CTS/SBA-15 scaffolds promoted alveolar bone regeneration in a rat model of periodontitis. To our knowledge, this is the first report that MET-loaded β-TCP/CTS/SBA-15 scaffolds have a positive effect on alveolar bone regeneration in periodontitis. Our findings might provide a new and promising strategy for repairing alveolar bone defects under the condition of periodontitis.
牙周炎是一种进行性感染性炎症疾病，会导致牙槽骨吸收和牙周附着丧失。因此，我们急需开发一种治疗性支架来修复牙周炎的牙槽骨缺损。在本研究中，我们通过冷冻干燥法设计了一种新型载药（二甲双胍，MET）复合支架，该支架由β-磷酸三钙（β-TCP）、壳聚糖（CTS）和介孔硅（SBA-15）组成。该支架有望结合 CTS 的优异生物相容性、β-TCP 的良好生物活性和 MET 的抗炎特性。体外实验结果表明，载 MET 的β-TCP/CTS/SBA-15 支架具有良好的细胞黏附性、合适的孔隙率和良好的生物相容性。将该 MET 复合支架植入牙周炎大鼠的牙槽骨缺损区，12 周后进行 Micro-CT 和组织学分析，以评估不同程度的愈合和矿化。结果表明，载 MET 的β-TCP/CTS/SBA-15 支架促进了牙周炎大鼠的牙槽骨再生。据我们所知，这是首次报道载 MET 的β-TCP/CTS/SBA-15 支架对牙周炎牙槽骨再生具有积极作用。我们的研究结果可能为牙周炎条件下修复牙槽骨缺损提供一种新的、有前景的策略。
