Department of Life science Engineering, Faculty of New Sciences and Technologies University of Tehran, P.O. Box 14395-1561, Tehran, Iran.
Department of Life science Engineering, Faculty of New Sciences and Technologies University of Tehran, P.O. Box 14395-1561, Tehran, Iran.
Carbohydr Polym. 2017 Feb 10;157:695-703. doi: 10.1016/j.carbpol.2016.10.023. Epub 2016 Oct 13.
New bioactive nanocomposite scaffolds from chitosan, sulfonated chitosan, and poly(ε-caprolactone) nanofibers were prepared. In this study, sulfonated chitosan was prepared using chlorosulfonic acid and combined with chitosan in order to improve the bioactivity of chitosan-based scaffolds. Meanwhile, different amounts of chopped-hydrophilic PCL nanofibers were incorporated into the fabricated scaffold in order to mimic extracellular matrix-like structure. In the end, apatite formed on/in the composite scaffolds through alternate dipping in calcium and phosphate rich solutions. The morphology, apatite forming ability, and mechanical properties of the scaffolds were evaluated. The results indicated that larger pore sizes, higher porosity and apatite deposition are obtained as a result of adding more PCL nanofibers. Contrary to the effects of increased pore size, addition of PCL nanofibers enhanced the mechanical properties. Cell culture experiments with osteoblast-like cells on the scaffolds demonstrated that incorporation of PCL nanofibers into the fabricated scaffolds improves cell viability and attachment.
采用氯磺酸制备了壳聚糖的磺化衍生物，并将其与壳聚糖复合，以提高壳聚糖基支架的生物活性。同时，将不同量的短切亲水性聚己内酯纳米纤维掺入到制备的支架中，以模拟细胞外基质样结构。最终，通过在钙和磷酸盐富溶液中交替浸泡，在复合支架上形成了磷灰石。评估了支架的形态、形成磷灰石的能力和力学性能。结果表明，随着添加更多的 PCL 纳米纤维，支架的孔径增大、孔隙率和磷灰石沉积增加。与孔径增大的影响相反，添加 PCL 纳米纤维增强了力学性能。在支架上进行成骨样细胞的细胞培养实验表明，将 PCL 纳米纤维掺入制备的支架中可提高细胞活力和黏附性。
