Vaghela Ravikumar, Arkudas Andreas, Steiner Dominik, Heltmann-Meyer Stefanie, Horch Raymund E, Hessenauer Maximilian
Department of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich-Alexander University of Erlangen-Nürnberg (FAU), Erlangen, Germany.
Microcirculation. 2022 Feb;29(2):e12742. doi: 10.1111/micc.12742. Epub 2021 Dec 10.
Transplantation of prefabricated tissue-engineered flaps can be a potential alternative for healing large tissue defects. Providing adequate vascular supply for an engineered tissue construct is one of the key points in establishing successful tissue engineering-based treatment approaches. In tissue engineering-based vascularization techniques like the arteriovenous loop, vascular grafts with high angiogenic potential can help to enhance neovascularization and tissue formation. Therefore, our study aimed to compare the angiogenic potential of vascular grafts from different locations in the rat.
The angiogenic activity was investigated by an ex vivo vessel outgrowth ring assay using 1-mm height vascular segments embedded in fibrin for 2 weeks.
Maximum vessel outgrowth was observed on Days 10-12. Upper extremity vessels exhibited stronger outgrowth than lower extremity vessels. Moreover, arterial vessels demonstrated higher angiogenic potential compared with venous vessels.
Collectively, our ex vivo findings suggest that upper extremity arterial vessels have a higher angiogenic capacity, which could be used to improve neovascularization and tissue formation in tissue engineering.
预制组织工程皮瓣移植可能是修复大面积组织缺损的一种潜在替代方法。为工程化组织构建体提供充足的血管供应是建立成功的基于组织工程的治疗方法的关键要点之一。在基于组织工程的血管化技术（如动静脉环）中，具有高血管生成潜力的血管移植物有助于增强新生血管形成和组织形成。因此，我们的研究旨在比较大鼠不同部位血管移植物的血管生成潜力。
通过体外血管生长环试验研究血管生成活性，该试验使用嵌入纤维蛋白中的1毫米高血管段培养2周。
在第10 - 12天观察到最大血管生长。上肢血管的生长比下肢血管更强。此外，与静脉血管相比，动脉血管表现出更高的血管生成潜力。
总体而言，我们的体外研究结果表明，上肢动脉血管具有更高的血管生成能力，可用于改善组织工程中的新生血管形成和组织形成。
