莪术总黄酮联合碳酸钙通过调控 Wnt3a/β-连环蛋白通路改善大鼠实验性骨质疏松症所致骨丢失。
Rhizoma drynariae total flavonoids combined with calcium carbonate ameliorates bone loss in experimentally induced Osteoporosis in rats via the regulation of Wnt3a/β-catenin pathway.
机构信息
Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, Sichuan, China.
Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, Sichuan, China.
出版信息
J Orthop Surg Res. 2021 Dec 4;16(1):702. doi: 10.1186/s13018-021-02842-3.
BACKGROUND
Rhizoma drynariae, a traditional Chinese herb, is commonly used in treatment of bone healing in osteoporotic fractures. However, whether the Rhizoma drynariae total flavonoids (RDTF) can promote the absorption of calcium and enhance the bone formation is unclear. The aim of the present study was to investigate the preventive effects of RDTF combined with calcium carbonate (CaCO) on estrogen deficiency-induced bone loss.
METHODS
Three-month-old Sprague-Dawley rats were ovariectomized (OVX) and then treated with CaCO RDTF, and their admixtures for ten weeks, respectively. The bone trabecular microstructure, bone histopathological examination, and serum biomarkers of bone formation and resorption were determined in the rat femur tissue. The contents of osteoprotegerin (OPG), receptor activator of the NF-κB (RANK), and its ligand (RANKL) in marrow were analyzed by ELISA, and the protein expressions of Wnt3a, β-catenin, and phosphorylated β-catenin (p-β-catenin) were analyzed by Western blot. Statistical analysis was conducted by using one-way analysis of variance (ANOVA) followed by LSD post hoc analysis or independent samples t test using the scientific statistic software SPSS version 20.0 RESULTS: RDTF combined with CaCO could promote osteosis and ameliorate bone loss to improve the repair of cracked bone trabeculae of OVX rats. Furthermore, RDTF combined with CaCO also could prevent OVX-induced decrease in collagen fibers in the femoral tissue of ovariectomized rats and promote the regeneration of new bone or cartilage tissue, while CaCO supplementation promoted the increase in bone mineral content. Nevertheless, there was no difference in the expression of Wnt3a, β-catenin and p-β-catenin between osteopenic rats and RDTF treated rats, but RDTF combined with CaCO could activate the Wnt3a/β-catenin pathway.
CONCLUSIONS
RDTF combined with CaCO could ameliorate estrogen deficiency-induced bone loss via the regulation of Wnt3a/β-catenin pathway.
背景
中药骨碎补常用于骨质疏松性骨折的骨愈合治疗。然而，骨碎补总黄酮（RDTF）是否能促进钙吸收并增强骨形成尚不清楚。本研究旨在探讨 RDTF 联合碳酸钙（CaCO）对去势诱导骨丢失的预防作用。
方法
3 月龄 Sprague-Dawley 大鼠去势后分别给予 CaCO、RDTF 及二者混合物治疗 10 周。检测大鼠股骨组织的骨小梁微结构、骨组织病理检查及血清骨形成和骨吸收标志物。ELISA 法检测骨髓中核因子κB 受体激活剂（RANK）及其配体（RANKL）、骨保护素（OPG）的含量，Western blot 法检测 Wnt3a、β-连环蛋白（β-catenin）及其磷酸化形式（p-β-catenin）的蛋白表达。采用单因素方差分析（ANOVA），LSD 事后分析或独立样本 t 检验进行统计学分析，统计软件采用 SPSS 20.0 版。
结果
RDTF 联合 CaCO 可促进成骨和改善骨丢失，以改善去势大鼠骨小梁裂缝的修复。此外，RDTF 联合 CaCO 还可以预防去势大鼠股骨组织胶原纤维减少，促进新骨或软骨组织再生，而 CaCO 补充可促进骨矿物质含量增加。然而，骨质疏松大鼠与 RDTF 治疗大鼠的 Wnt3a、β-catenin 和 p-β-catenin 表达无差异，但 RDTF 联合 CaCO 可激活 Wnt3a/β-catenin 通路。
结论
RDTF 联合 CaCO 可通过调节 Wnt3a/β-catenin 通路改善去势诱导的骨丢失。
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