First Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan, China.
Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China.
Front Endocrinol (Lausanne). 2022 Aug 2;13:930912. doi: 10.3389/fendo.2022.930912. eCollection 2022.
Osteoporosis is increasingly becoming a serious problem affecting the quality of life of the older population. Several experimental studies have shown that Chinese medicine has a definite effect on improving osteoporosis. Based on transcriptome sequencing, we analyzed the differential gene expression and mechanism of the related signaling pathways. Fifteen rats were randomly divided into an experimental group, a model group, and a sham surgery group. The rat model for menopausal osteoporosis was established using an ovariectomy method. One week after modeling, the experimental group was administered(intragastric administration)8.1 g/kg of , whereas the model and sham groups received 0.9% saline solution twice daily for 12 weeks. Subsequently, the rats were sacrificed, and the left femur of each group was removed for computerized tomography testing, while right femurs were used for hematoxylin and eosin staining. High-throughput RNA sequencing and functional and pathway enrichment analyses were performed. Comparing the gene expression between the experimental and model groups, 149 differential genes were identified, of which 44 were downregulated and 105 were upregulated. The criteria for statistical significance were |log2 Fold Change| > 1 and < 0.05. Gene ontology analysis showed that the differentially expressed genes were enriched in cell component terms such as cell part and outer cell membrane part, and the genes were associated with cell process, biological regulation, metabolic processes, DNA transcription, and catalytic activity. Enrichment analysis of Kyoto Encyclopedia of Genes and Genomes pathways showed significantly enriched pathways associated with systemic lupus erythematosus, herpes simplex infection, circadian rhythm, vascular smooth muscle contraction, the AGE-RAGE signaling pathway in diabetic complications, and the TNF, Apelin, and Ras signaling pathways. Our results revealed that the Npas2, Dbp, Rt1, Arntl, Grem2, H2bc9, LOC501233, Pla2g2c, Hpgd, Pde6c, and Dner genes, and the circadian rhythm, lipid metabolism, inflammatory signaling pathway, and immune pathways may be the key targets and pathways for traditional Chinese medicine therapy of Rhizoma Drynariae in osteoporosis.
骨质疏松症日益成为影响老年人口生活质量的严重问题。多项实验研究表明，中医药对改善骨质疏松症有确切疗效。基于转录组测序，我们分析了相关信号通路的差异基因表达和机制。将 15 只大鼠随机分为实验组、模型组和假手术组。采用卵巢切除术方法建立绝经后骨质疏松症大鼠模型。建模后 1 周，实验组给予 8.1 g/kg 的 灌胃，模型组和假手术组每日给予 0.9%生理盐水 2 次，共 12 周。随后处死大鼠，取出各组左侧股骨进行计算机断层扫描检测，右侧股骨用于苏木精-伊红染色。进行高通量 RNA 测序和功能及通路富集分析。比较实验组和模型组之间的基因表达，共鉴定出 149 个差异基因，其中 44 个下调，105 个上调。统计显著性标准为 |log2 Fold Change| > 1 和 < 0.05。基因本体论分析显示，差异表达基因在细胞成分术语中富集，如细胞部分和外细胞膜部分，基因与细胞过程、生物调节、代谢过程、DNA 转录和催化活性相关。京都基因与基因组百科全书通路富集分析显示，与系统性红斑狼疮、单纯疱疹感染、昼夜节律、血管平滑肌收缩、糖尿病并发症中的 AGE-RAGE 信号通路以及 TNF、Apelin 和 Ras 信号通路相关的显著富集通路。我们的研究结果表明，Npas2、Dbp、Rt1、Arntl、Grem2、H2bc9、LOC501233、Pla2g2c、Hpgd、Pde6c 和 Dner 基因，以及昼夜节律、脂质代谢、炎症信号通路和免疫通路可能是中药治疗骨质疏松症的关键靶点和通路。
