College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China; University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, Guangzhou 510642, China.
College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China; University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, Guangzhou 510642, China.
Sci Total Environ. 2022 Feb 20;808:152098. doi: 10.1016/j.scitotenv.2021.152098. Epub 2021 Dec 2.
Polystyrene nanoplastics (PS-NPs) can impair antioxidant, immune, and nervous system functions as well as growth and development in aquatic organisms. At present, however, little is known about the effects and underlying mechanisms of PS-NPs on the digestive system of marine fish. Here, we studied the effects of these plastics on the intestinal health and growth performance of juvenile orange-spotted groupers (Epinephelus coioides). Based on histopathological analysis, we found that the liver and intestines can uptake PS-NPs at exposure concentrations of 300 and 3000 μg/ml, respectively. After 14 d of exposure, the activities of digestive enzymes lipase (LPS), trypsin (TRS), and lysozyme (LZM) were reduced, indicating that PS-NPs negatively affected digestive function in juvenile groupers. The PS-NPs also altered microbial community composition, resulting in a decrease in diversity and simplification of network relationships in the intestinal microbiota, but a significant increase in certain harmful bacteria, especially Vibrio and Aliivibrio. In addition, community assembly changed from being driven primarily by deterministic processes (68.89% for control group) to stochastic processes (73.33% and 51.11% for 300 and 3000 μg/ml PS-NP exposure groups, respectively). Furthermore, the specific growth rate (SGR) of the juvenile orange-spotted groupers decreased significantly with increasing PS-NP exposure concentrations (0.158% ± 0.032%, 0.095% ± 0.020%, and 0.074% ± 0.016% for 0, 300, and 3000 μg/L PS-NP groups, respectively). These results suggest that marine PS-NPs are harmful to the digestive system of juvenile fish and highlight the importance of evaluating the long-term impact of NPs in reshaping marine populations.
聚苯乙烯纳米塑料 (PS-NPs) 可损害水生生物的抗氧化、免疫和神经系统功能以及生长和发育。然而，目前对于 PS-NPs 对海洋鱼类消化系统的影响和潜在机制知之甚少。在这里，我们研究了这些塑料对幼年鞍带石斑鱼（Epinephelus coioides）肠道健康和生长性能的影响。基于组织病理学分析，我们发现肝脏和肠道可以分别在暴露浓度为 300 和 3000μg/ml 时摄取 PS-NPs。暴露 14 天后，脂肪酶 (LPS)、胰蛋白酶 (TRS) 和溶菌酶 (LZM) 的消化酶活性降低，表明 PS-NPs 对幼年石斑鱼的消化功能有负面影响。PS-NPs 还改变了微生物群落组成，导致肠道微生物群多样性降低，网络关系简化，但某些有害细菌，特别是弧菌和阿里氏菌显著增加。此外，群落组装从主要由确定性过程驱动（对照组为 68.89%）转变为随机过程（300 和 3000μg/ml PS-NP 暴露组分别为 73.33%和 51.11%）。此外，随着 PS-NP 暴露浓度的增加，幼年鞍带石斑鱼的特定生长率（SGR）显著降低（0、300 和 3000μg/L PS-NP 组分别为 0.158%±0.032%、0.095%±0.020%和 0.074%±0.016%）。这些结果表明，海洋 PS-NPs 对幼年鱼类的消化系统有害，并强调了评估 NPs 重塑海洋种群的长期影响的重要性。
