CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, PR China; College of Engineering, Northeast Agriculture University, Harbin 150030, PR China.
CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, PR China.
Bioresour Technol. 2022 Feb;345:126483. doi: 10.1016/j.biortech.2021.126483. Epub 2021 Dec 2.
The understanding and enrichment of consortia formed by syntrophic butyrate-oxidizing bacteria and methanogens in the complex environment are crucial for effectively degrading butyrate and preventing acid inhibition. In this study, the better butyrate-tolerated and highly efficient microbial consortia were domesticated and enriched through adding butyric acid ranging from 0.2 to 4.4 g/(L·d). The volumetric biogas production continuously increased to 1.65 L/(L·d). Microbial community diversity showed that a dramatic shift of bacterial structure occurred at BAL of 1.6 g/(L·d) and the structure presented better stability at high BAL. The syntrophic consortia and the main metabolic pathways were revealed through combination of the 16S rDNA and metagenome sequencing analyses. Syntrophomonas was the major butyrate-oxidizing bacterium and oxidized butyrate mainly through β-oxidaiton. Synergistaceae and Mesotoga acted as the main acetate-oxidizing bacteria. IHT and methanogenesis pathways were strongly enhanced by DMER64 and Methanosarcina as the main H carrier and dominant methanogen, respectively.
在复杂环境中，理解和丰富丁酸氧化细菌和产甲烷菌形成的共生体对于有效降解丁酸和防止酸抑制至关重要。在这项研究中，通过添加丁酸（0.2 至 4.4 g/（L·d）），驯化和富集了具有更好丁酸耐受性和高效的微生物共生体。体积沼气产量持续增加至 1.65 L/（L·d）。微生物群落多样性表明，在 BAL 为 1.6 g/（L·d）时细菌结构发生了剧烈变化，并且在高 BAL 下结构呈现出更好的稳定性。通过 16S rDNA 和宏基因组测序分析的结合，揭示了共生体和主要代谢途径。Syntrophomonas 是主要的丁酸氧化细菌，主要通过β-氧化丁酸。协同菌科和中温甲烷菌作为主要的乙酸氧化细菌。IHT 和产甲烷途径分别通过 DMER64 和产甲烷菌（主要的 H 载体和优势产甲烷菌）得到了强烈增强。
