College of Chemistry and Chemical Engineering, Qiqihar University, No.42 Wenhua Street, Qiqihar 161006, China.
College of Chemistry and Chemical Engineering, Qiqihar University, No.42 Wenhua Street, Qiqihar 161006, China.
Biophys Chem. 2022 Feb;281:106728. doi: 10.1016/j.bpc.2021.106728. Epub 2021 Nov 25.
The fabrication of cell models containing artificial cytosol is challenging. Herein we constructed an artificial cytosol contained cell model by electroformation method. Agarose was selected as the main component of the artificial cytosol, and sucrose was added into the agarose to regulate the sol viscosity and the phase transition temperature. The viscosity of the sol with the mass ratio (agarose-sucrose) 1:9 was closest to the natural cytosol. DSPC/20 mol% cholesterol was used to form large unilamellar vesicles (LUVs) as cell model compartment. The rhodamine release experiment confirmed that the unique release profile of agarose-sucrose@LUVs is suitable as a drug carrier. Doxorubicin is loaded in the agarose-sucrose@LUVs, and their half maximum inhibition concentration on HeLa cells is 0.016 μmol L, which means 28.7 times increase in inhibition efficiency over free doxorubicin.
人工细胞浆的细胞模型的构建具有挑战性。在此，我们通过电成型法构建了一种含有人工细胞浆的细胞模型。琼脂糖被选为人工细胞浆的主要成分，并向琼脂糖中添加蔗糖以调节溶胶的粘度和相转变温度。质量比（琼脂糖-蔗糖）为 1:9 的溶胶的粘度最接近天然细胞浆。DSPC/20mol%胆固醇用于形成大单层囊泡（LUVs）作为细胞模型隔室。罗丹明释放实验证实，琼脂糖-蔗糖@LUVs 的独特释放谱适合作为药物载体。阿霉素装载在琼脂糖-蔗糖@LUVs 中，它们对 HeLa 细胞的半最大抑制浓度为 0.016μmol L，这意味着与游离阿霉素相比，抑制效率提高了 28.7 倍。
