采用尿酸酶封装纳米花辅助便携式电化学尿酸计对尿液样本中尿酸进行连续、准确的家庭监测。
Continual and accurate home monitoring of uric acid in urine samples with uricase-packaged nanoflowers assisted portable electrochemical uricometer.
机构信息
Engineering Research Center of Bio-process, MOE, School of Food and Biological Engineering, Intelligent Manufacturing Institute, Hefei University of Technology, Hefei, 230009, China.
Anhui Province Institute of Product Quality Supervision & Inspection, Hefei, 230051, PR China.
出版信息
Biosens Bioelectron. 2022 Feb 15;198:113804. doi: 10.1016/j.bios.2021.113804. Epub 2021 Nov 17.
A convenient, fast and non-invasive portable electrochemical uricometer (PUM) assisted with the uricase-packaged nanoflowers (NFs) was constructed for continually and accurately monitoring of uric acid (UA) in urine samples at random intervals in just 20 s. Only a small amount of urine (50 μL) was needed for each test. Electrochemical deposition was adopted to modify gold nanoparticles (AuNPs) on screen-printed carbon electrodes (SPCE) and uricase-inorganic hybrid NFs (UOx-NFs) induced by calcium ions (Ca) were introduced for UA detection with expected specificity. Cyclic voltammetry (CV) (detection limit of 8.87 μM and liner range of 0-4 mM) and amperometry (detection limit of 0.82 μM and liner range of 0-5 mM) protocols were studied for UA detection, respectively. Finally, the uric acid in urine had be successfully continually monitored from volunteers with various dietary choosing, the results of which can be adopted as the effective evidence for uric acid control.
构建了一种便捷、快速、非侵入式的便携式电化学尿酸计（PUM），该尿酸计辅助尿酸酶封装的纳米花（NFs），可在 20 秒内以随机间隔持续、准确地监测尿液样本中的尿酸（UA）。每次检测仅需 50 μL 的少量尿液。采用电化学沉积法在丝网印刷碳电极（SPCE）上修饰金纳米粒子（AuNPs），并引入钙离子（Ca）诱导的尿酸酶-无机杂化 NFs（UOx-NFs），以实现 UA 的预期特异性检测。分别采用循环伏安法（CV）（检测限为 8.87 μM，线性范围为 0-4 mM）和安培法（检测限为 0.82 μM，线性范围为 0-5 mM）对 UA 进行检测。最后，成功地对志愿者的尿液中的尿酸进行了持续监测，其结果可作为尿酸控制的有效依据。
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