School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China.
Mikrochim Acta. 2024 Aug 7;191(9):521. doi: 10.1007/s00604-024-06585-w.
MoO NPs was rapidly synthesized at room temperature by an easy stirring method. It was interesting to find that MoO NPs induce OH to generate active free radicals (ROS), which is a highly promising property in chemiluminescence (CL). Benefiting from the abundant oxygen vacancy, MoO NPs adsorbs HO and turn it into ·OH. The oxidase activity of fluorescein under visible light had already been reported, which catalyzes dissolved oxygen to become O· and continue to convert to HO. By creating the synergy effect with fluorescein, MoO NPs strengthen the CL intensity of K[Fe(CN)]-fluorescein system significantly. Utilizing the quench effect of uric acid for the CL intensity, we developed a rapid, simple, and highly sensitive CL platform for uric acid detection. The linear range was 5-80 µM and the detection limit (LOD) for uric acid was 3.11 µM (S/N = 3). This work expanded the application of MoO NPs in the CL field and developed a simple and highly sensitive CL sensing system to detect UA in human saliva.
MoO NPs 可通过简单的搅拌法在室温下快速合成。有趣的是，MoO NPs 能诱导 OH 生成活性自由基（ROS），这在化学发光（CL）中是一个很有前途的特性。得益于丰富的氧空位，MoO NPs 吸附 HO 并将其转化为·OH。荧光素在可见光下的氧化酶活性已被报道，它能催化溶解氧生成 O·，并继续转化为 HO。通过与荧光素产生协同效应，MoO NPs 显著增强了 K[Fe(CN)]-荧光素体系的 CL 强度。利用尿酸对 CL 强度的猝灭效应，我们开发了一种快速、简单、高灵敏度的尿酸检测 CL 平台。线性范围为 5-80 μM，尿酸的检测限（LOD）为 3.11 μM（S/N = 3）。本工作扩展了 MoO NPs 在 CL 领域的应用，并开发了一种简单、高灵敏度的 CL 传感系统，用于检测人唾液中的 UA。
