Veerhuis R, Klar-Mohamad N, van Es L A, Daha M R
Scand J Immunol. 1986 May;23(5):581-7. doi: 10.1111/j.1365-3083.1986.tb01991.x.
Expression of C1q receptors on the plasma membrane of thioglycollate-stimulated guinea pig peritoneal exudate macrophages increased 1.54 times as compared to unstimulated controls. A Scatchard plot of the binding of 125I-C1q to the cells revealed that the binding is a result of an increase in the number of receptors and not to an increased affinity of the receptors. Thioglycollate-activated macrophages were found to be 1.6 times more active than nonactivated macrophages in the binding of 125I-C1q at 4 degrees C. The enhanced binding of 125I-C1q by activated peritoneal macrophages was reflected in an increase in the amount of 125I-C1q degraded by these cells as compared to resident peritoneal macrophages. This suggests that stimulation of phagocytic cells leads to an increase in the expression of C1q receptors and to a concomitant increase in the uptake and degradation of C1q.
与未刺激的对照相比，巯基乙酸盐刺激的豚鼠腹腔渗出液巨噬细胞质膜上C1q受体的表达增加了1.54倍。125I-C1q与细胞结合的Scatchard图显示，这种结合是受体数量增加的结果，而不是受体亲和力增加的结果。发现在4℃下，巯基乙酸盐激活的巨噬细胞在结合125I-C1q方面比未激活的巨噬细胞活跃1.6倍。与驻留腹腔巨噬细胞相比，激活的腹腔巨噬细胞对125I-C1q结合的增强反映在这些细胞降解的125I-C1q量的增加上。这表明吞噬细胞的刺激导致C1q受体表达增加，并伴随C1q摄取和降解的增加。
