产油酵母菌株CGMCC 2.1609的基因组序列
Genome sequence of the oleaginous yeast strain CGMCC 2.1609.
作者信息
Sambles Christine, Middelhaufe Sabine, Soanes Darren, Kolak Dagmara, Lux Thomas, Moore Karen, Matoušková Petra, Parker David, Lee Rob, Love John, Aves Stephen J
机构信息
Biosciences, College of Life and Environmental Sciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, UK.
出版信息
Genom Data. 2017 May 15;13:1-2. doi: 10.1016/j.gdata.2017.05.009. eCollection 2017 Sep.
Abstract
Most eukaryotic oleaginous species are yeasts and among them the basidiomycete red yeast, () (Pucciniomycotina) is known to produce high quantities of lipids when grown in nitrogen-limiting media, and has potential for biodiesel production. The genome of the CGMCC 2.1609 strain of this oleaginous red yeast was sequenced using a hybrid of Roche 454 and Illumina technology generating 13 × coverage. The assembly was carried out using MIRA and scaffolded using MAQ and BAMBUS. The sequencing and assembly resulted in 365 scaffolds with total genome size of 33.4 Mb. The complete genome sequence of this strain was deposited in GenBank and the accession number is LKER00000000. The annotation is available on Figshare (doi:10.6084/m9.figshare.4754251).
摘要
大多数真核产油物种是酵母，其中担子菌红酵母（ ）（ Pucciniomycotina）在氮限制培养基中生长时已知会产生大量脂质，并且具有生物柴油生产潜力。使用罗氏454和Illumina技术的混合方法对这种产油红酵母的CGMCC 2.1609菌株基因组进行了测序，覆盖率达13倍。使用MIRA进行组装，并使用MAQ和BAMBUS进行支架构建。测序和组装产生了365个支架，基因组总大小为33.4Mb。该菌株的完整基因组序列已存入GenBank，登录号为LKER00000000。注释可在Figshare上获取（doi:10.6084/m9.figshare.4754251）。
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