Wang Wei, Li Qing-Ling, Ma Qiang, Xia Ran, Gao Bing, Wang Yi, Wang Jing
Clinical College of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, China.
Clinical College of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, China; Graduate School, Anhui University of Chinese Medicine, Hefei 230012, China.
Zhen Ci Yan Jiu. 2021 Nov 25;46(11):935-41. doi: 10.13702/j.1000-0607.201219.
To observe the effect of moxibustion combined with benazepril on cardiac function and expression levels of myocardial interleukin-18(IL-18), phosphorylated protein kinase B(p-Akt) in rats with chronic heart failure (CHF), so as to explore its underlying mechanisms in improvement of CHF.
Fifty male rats were randomly divided into normal, model, moxibustion, benazepril and moxibustion+benazepril groups (=10 rats per group). The CHF model was established by intraperitoneal injection of doxorubicin hydrochloride solution (DOX, 2.5 mg/kg) twice a week for 4 weeks. After successful modeling, the rats in the normal and model groups were fed with normal diet, and fixed on a rat plate for 20 min each time without any treatment. Mild moxibustion was applied to bilateral "Feishu" (BL13) and "Xinshu" (BL15) for 20 min each time, for 3 weeks in the moxibustion and moxibustion+benazepril groups. Rats of the benazepril and moxibustion+benazepril groups received gavage of benazepril (2 mg/kg) once daily for 3 weeks. The general behaviors of rats were observed. The ejection fraction (EF), left ventricular diameter shortening (FS), left ventricular end-diastolic diameter (LVIDd), left ventricular end-systolic diameter (LVIDs), heart rate (HR) and ventricular septal thickness (IVS) were examined by echocardiography. The content of serum N-terminal pro-brain natriuretic peptide (NT-proBNP) was detected by enzyme-linked immunosorbent assay, and expression levels of myocardial IL-18, p-Akt were detected by Western blot.
Compared with the normal group, the EF, FS, IVS, and myocardial p-Akt expression level were significantly reduced (<0.01), and the LVIDd, LVIDs, HR, and serum NT-proBNP content and myocardial IL-18 expression level were significantly increased in the model group (<0.01). In comparison with the model group, the EF, FS, IVS, and myocardial p-Akt were remarkably up-regulated (<0.05, <0.01), and the LVIDd, LVIDs, HR, serum NT-proBNP content, and myocardial IL-18 expression level were significantly down-regulated (<0.05, <0.01) in the moxibustion, benazepril, and moxibustion+benazepril groups. Compared with the moxibustion+benazepr group, the levels of LVIDs, HR, serum NT-proBNP and myocardial IL-18 expression were obviously higher (<0.05, <0.01), while the levels of EF, FS, IVS and p-Akt were significantly lower in the moxibustion and benazepril groups (<0.01).
Moxibustion combined with benazepril improves cardiac function in CHF rats, and is superior to simple moxibustion and simple benazepril in reducing IL-18 expression and increasing p-Akt expression in myocardial tissue.
观察艾灸联合贝那普利对慢性心力衰竭（CHF）大鼠心功能及心肌白细胞介素 - 18（IL - 18）、磷酸化蛋白激酶B（p - Akt）表达水平的影响，以探讨其改善CHF的潜在机制。
将50只雄性大鼠随机分为正常组、模型组、艾灸组、贝那普利组和艾灸 + 贝那普利组（每组10只）。采用腹腔注射盐酸多柔比星溶液（DOX，2.5 mg/kg），每周2次，连续4周建立CHF模型。造模成功后，正常组和模型组大鼠给予正常饮食，每次固定于鼠板20分钟，不做任何处理。艾灸组和艾灸 + 贝那普利组大鼠双侧“肺俞”（BL13）和“心俞”（BL15）轻度艾灸，每次20分钟，共3周。贝那普利组和艾灸 + 贝那普利组大鼠每日灌胃贝那普利（2 mg/kg），共3周。观察大鼠一般行为。采用超声心动图检测射血分数（EF）、左心室直径缩短率（FS）、左心室舒张末期内径（LVIDd）、左心室收缩末期内径（LVIDs）、心率（HR）和室间隔厚度（IVS）。采用酶联免疫吸附测定法检测血清N末端脑钠肽前体（NT - proBNP）含量，采用蛋白质免疫印迹法检测心肌IL - 18、p - Akt表达水平。
与正常组比较，模型组EF、FS、IVS及心肌p - Akt表达水平显著降低（<0.01），LVIDd、LVIDs、HR、血清NT - proBNP含量及心肌IL - 18表达水平显著升高（<0.01）。与模型组比较，艾灸组、贝那普利组和艾灸 + 贝那普利组EF、FS、IVS及心肌p - Akt显著上调（<0.05，<0.01），LVIDd、LVIDs、HR、血清NT - proBNP含量及心肌IL - 18表达水平显著下调（<0.05，<0.01）。与艾灸 + 贝那普利组比较，艾灸组和贝那普利组LVIDs、HR、血清NT - proBNP及心肌IL - 18表达水平明显升高（<0.05，<0.01），而EF、FS、IVS及p - Akt水平显著降低（<0.01）。
艾灸联合贝那普利可改善CHF大鼠的心功能，在降低心肌组织IL - 18表达和增加p - Akt表达方面优于单纯艾灸和单纯贝那普利治疗。
