Wang Jing, Zeng Yong-Lei, Wu Feng-Qin, Sun Rui-Rui, Chen Jing, Jia Xue-Zhao, Xi Yu-Hong
Clinical College of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230038, China; 1 The Second Affiliated Hospital, Anhui University of Chinese Medicine, Hefei 230061; 2 Graduate School, Anhui University of Chinese Medicine, Hefei 230038.
Clinical College of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230038, China; The Second Affiliated Hospital, Anhui University of Chinese Medicine, Hefei 230061.
Zhen Ci Yan Jiu. 2016 Oct 25;41(5):429-34.
To observe the effect of moxibustion stimulation of "Feishu" (BL 13) and "Xinshu" (BL 15) on pathological changes of myocardium and the expression of myocardial myeloid differentiation factor 88 (MyD 88) protein and Caspase 3 mRNA in chronic heart failure (CHF) rats, so as to explore its mechanism underlying improvement of CHF.
SD male rats were randomly divided into normal (=9), model (=8), moxibustion (=8), medication (=8) and moxibustion + medication (=8) groups. In addition, the other 6 rats (3/normal and 3/model groups) were used for measuring cardiac ventricle weight and H.E. stain. The CHF model was made by intraperitoneal injection of Adriamycin (ADR, from 1 to 4 mg/kg, once every other day for 15 days). Mild moxibustion was applied to bilateral BL13 and BL15 for 15 min, once daily for 3 weeks. Rats of the medication group were treated by Captopril (gavage) for 3 weeks. The expression of myocardial Caspase 3 mRNA and MyD 88 protein of the left ventricle was determined by quantitative real time-PCR and Western blot, respectively.
In comparison with the normal group, the myocardial damage (cell swelling, cytoplasma vaculation, and disordered arrangement, rupture and lysis of some cardiac muscle fibers), and the expression levels of myocardial MyD 88 protein and Caspase 3 mRNA were obviously increased in the model group(<0.01). After the interventions, the myocardial damage was relatively milder, and the expression of myocardial MyD 88 protein and Caspase 3 mRNA were significantly down-regulated in the moxibustion, me-dication and moxibustion+medication groups in comparison with the model group(<0.05). No significant differences were found among the 3 treatment groups in the expression levels of MyD 88 protein and Caspase 3 mRNA(>0.05).
Moxibustion intervention can suppress CHF induced up-regulation of expression of myocardial MyD 88 protein and Caspase 3 mRNA in rats, which may contribute to its effect in relieving myocardial injury.
观察艾灸“肺俞”（BL 13）和“心俞”（BL 15）对慢性心力衰竭（CHF）大鼠心肌病理变化及心肌髓样分化因子88（MyD 88）蛋白和Caspase 3 mRNA表达的影响，以探讨其改善CHF的作用机制。
将雄性SD大鼠随机分为正常组（n = 9）、模型组（n = 8）、艾灸组（n = 8）、药物组（n = 8）和艾灸+药物组（n = 8）。另外，取6只大鼠（正常组和模型组各3只）用于测量心室重量及进行苏木精-伊红（H.E.）染色。采用腹腔注射阿霉素（ADR，1～4 mg/kg，隔日1次，共15天）制备CHF模型。对双侧BL13和BL15进行温和艾灸15分钟，每日1次，共3周。药物组大鼠采用卡托普利灌胃治疗3周。分别采用实时定量聚合酶链反应（PCR）和蛋白质免疫印迹法检测左心室心肌Caspase 3 mRNA和MyD 88蛋白的表达。
与正常组比较，模型组心肌损伤（细胞肿胀、胞浆空泡化、部分心肌纤维排列紊乱、断裂及溶解）及心肌MyD 88蛋白和Caspase 3 mRNA表达水平明显升高（P<0.01）。干预后，艾灸组、药物组和艾灸+药物组心肌损伤较模型组减轻，心肌MyD 88蛋白和Caspase 3 mRNA表达水平均较模型组显著下调（P<0.05）。3个治疗组MyD 88蛋白和Caspase 3 mRNA表达水平比较，差异无统计学意义（P>0.05）。
艾灸干预可抑制大鼠CHF时心肌MyD 88蛋白和Caspase 3 mRNA表达上调，这可能是其减轻心肌损伤的作用机制之一。
