艾灸双侧肺俞穴（BL13）和心俞穴（BL15）联合贝那普利对慢性心力衰竭大鼠心肌细胞细胞凋亡指数及凋亡相关蛋白细胞色素 c、凋亡诱导因子的影响。

Effects of moxibustion at bilateral Feishu (BL13) and Xinshu (BL15) combined with benazepril on myocardial cells apoptosis index and apoptosis-related proteins cytochrome c and apoptosis-inducing factor in rats with chronic heart failure.

机构信息

College of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, China.

Graduate school, Anhui University of Chinese Medicine, Hefei 230012, China.

出版信息

J Tradit Chin Med. 2022 Apr;42(2):227-233. doi: 10.19852/j.cnki.jtcm.20220126.001.

DOI:10.19852/j.cnki.jtcm.20220126.001

PMID:35473343

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9924675/

Abstract

OBJECTIVE

To observe the effects of moxibustion at bilateral Feishu (BL13) and Xinshu (BL15) combined with benazepril on myocardial cells apoptosis index, the expression levels of apoptosis-related proteins cytochrome c (Cyt-C) and apoptosis-inducing factor (AIF) in chronic heart failure (CHF) rats.

METHODS

Sixty-five rats were randomly divided into normal group () and model-I group (). After modeling, CHF rats in model-I group were divided into model group, moxibustion group, benazepril group, moxibustion plus benazepril group (abbreviated as aibei group, the same below), 10 rats in each group. Echocardiogram index was examined by echocardiography. Hemodynamic indices were measured by rat cardiac function meter. Serum B-type brain natriuretic peptide (BNP) was detected by enzyme-linked immunosorbent assay. Myocardial cells apoptosis index was detected by terminal-deoxynucleoitidyl transferase mediated nick end labeling staining. Pathological changes of myocardial tissues were observed by hematoxylin and eosin staining. The expression levels of Cyt-C and AIF in myocardial tissues were detected by Western blot.

RESULTS

Compared with normal group, ejection fraction and left ventricular diameter shortening rate in model-Ⅰ group were significantly reduced, myocardial cells of rats in model group exhibited unclear transverse striations, cells swellings and vacuoles, cardiac functions were deteriorated, serum BNP level, myocardial cells apoptosis index, and the expression levels of Cyt-C and AIF were significantly increased. Compared with model group, myocardial cells of rats in moxibustion group, benazepril group, and aibei group were dyed more evenly, muscle fibers were arranged relatively neatly, cardiac functions were improved, serum BNP level, myocardial cells apoptosis index, and the expression levels of Cyt-C and AIF were significantly decreased. Compared with aibei group, cardiac functions were worsened, myocardial cells apoptosis index, and the expression levels of Cyt-C and AIF were increased.

CONCLUSION

Moxibustion at bilateral Feishu (BL13) and Xinshu (BL15) combined with benazepril could improve CHF better than moxibustion at bilateral Feishu (BL13) and Xinshu (BL15) or benazepril alone. The mechanisms might be that they can inhibit the expressions of Cyt-C and AIF, and inhibit the apoptosis of cardiomyocytes.

摘要

目的

观察艾灸双侧肺俞（BL13）、心俞（BL15）联合贝那普利对慢性心力衰竭（CHF）大鼠心肌细胞细胞凋亡指数、细胞色素 c（Cyt-C）和凋亡诱导因子（AIF）表达水平的影响。

方法

65 只大鼠随机分为正常组（）和模型-I 组（）。造模后，模型-I 组 CHF 大鼠分为模型组、艾灸组、贝那普利组、艾灸+贝那普利组（简称 aibei 组，下同），每组 10 只。采用超声心动图仪检测各组大鼠的超声心动图指标，采用大鼠心脏功能仪检测各组大鼠的血流动力学指标，采用酶联免疫吸附试验检测各组大鼠血清 B 型脑钠肽（BNP）水平，采用末端脱氧核苷酸转移酶介导的缺口末端标记染色法检测心肌细胞凋亡指数，采用苏木精-伊红（HE）染色法观察各组大鼠心肌组织的病理变化，采用 Western blot 法检测各组大鼠心肌组织中 Cyt-C 和 AIF 的表达水平。

结果

与正常组比较，模型-I 组大鼠的射血分数和左心室短轴缩短率均明显降低，模型组大鼠心肌细胞横纹不清，细胞肿胀、有空泡，心功能恶化，血清 BNP 水平、心肌细胞凋亡指数及 Cyt-C 和 AIF 表达水平均明显升高；与模型组比较，艾灸组、贝那普利组和 aibei 组大鼠心肌细胞染色较均匀，肌纤维排列较整齐，心功能改善，血清 BNP 水平、心肌细胞凋亡指数及 Cyt-C 和 AIF 表达水平均明显降低；与 aibei 组比较，艾灸组大鼠心功能恶化，心肌细胞凋亡指数及 Cyt-C 和 AIF 表达水平升高。