犬淋巴瘤细胞系中趋化因子受体 7 的表达与功能分析。
Expression and functional analysis of chemokine receptor 7 in canine lymphoma cell lines.
机构信息
The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Laboratory of Molecular Diagnostics and Therapeutics, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8511, Japan.
出版信息
J Vet Med Sci. 2022 Jan 7;84(1):25-30. doi: 10.1292/jvms.21-0468. Epub 2021 Dec 3.
C-C chemokine receptor 7 (CCR7) contributes to cell homing to lymph nodes (LNs). Recent studies reported that CCR7 is also expressed in tumor cells, which correlates with LN metastasis in various cancers. However, the expression of CCR7 in tumor cells is unknown in dogs due to the lack of appropriate antibodies. In the present study, a fusion protein of C-C chemokine ligand 19 (CCL19) was employed as an alternative method to CCR7 antibodies. The fusion CCL19 protein specifically detected CCR7 expressed in canine lymphoma cell lines, which showed active chemotaxis to both canine and mouse ligands. The present study will help further research on the involvement of canine CCR7 in LN metastasis.
C-C 趋化因子受体 7（CCR7）有助于细胞归巢到淋巴结（LNs）。最近的研究报道，CCR7 也在肿瘤细胞中表达，这与各种癌症中的淋巴结转移相关。然而，由于缺乏合适的抗体，狗的肿瘤细胞中 CCR7 的表达情况尚不清楚。在本研究中，采用 C-C 趋化因子配体 19（CCL19）的融合蛋白作为 CCR7 抗体的替代方法。融合的 CCL19 蛋白特异性地检测到在犬淋巴瘤细胞系中表达的 CCR7，这些细胞系对犬和鼠配体均表现出活跃的趋化性。本研究将有助于进一步研究犬 CCR7 在 LN 转移中的作用。
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