Xiao Jingchuan, Zhang Yingai
Central Laboratory, Affiliated Haikou Hospital of Xiangya Medical College, Central South University, Haikou, Hainan, China.
School of Life Sciences, Hainan University, Haikou, Hainan, China.
Evol Bioinform Online. 2021 Nov 24;17:11769343211057589. doi: 10.1177/11769343211057589. eCollection 2021.
The Aurora kinases form a family of 3 genes encoding serine/threonine kinases and are involved in the regulation of cell division during the mitosis. This study was designed to investigate the prognostic role of Aurora kinases in hepatocellular carcinoma (HCC). In this study, we analyzed the expression, overall survival (OS) data, promoter methylation level, and relationship with immunoinhibitors of Aurora kinases in patients with HCC from GEPIA2, UALCAN, OncoLnc, and TISIDB databases. Protein-protein interaction (PPI) network, gene ontology, Kyoto Encyclopedia of Genes and Genomes (KEGG), and Reactome pathway analysis were performed using the STRING database and Cytoscape software. We found that the mRNA expression, stages of HCC, and OS of AURKA and AURKB in HCC tissues were significantly different from control tissues, but there were significant inconsistencies in promoter methylation level and relationship with immunoinhibitors for AURKA and AURKB. None of the above items were significantly different for AURKC. Furthermore, a hub module including AURKA, AURKB, and AURKC was identified within the PPI network constructed with the Molecular Complex Detection (MCODE) plug-in in Cytoscape software. Our results show that AURKB could be a potential biomarker for HCC prognosis.
极光激酶由3个编码丝氨酸/苏氨酸激酶的基因组成，参与有丝分裂期间细胞分裂的调控。本研究旨在探讨极光激酶在肝细胞癌（HCC）中的预后作用。在本研究中，我们从GEPIA2、UALCAN、OncoLnc和TISIDB数据库分析了HCC患者中极光激酶的表达、总生存（OS）数据、启动子甲基化水平以及与免疫抑制剂的关系。使用STRING数据库和Cytoscape软件进行了蛋白质-蛋白质相互作用（PPI）网络、基因本体、京都基因与基因组百科全书（KEGG）和Reactome通路分析。我们发现，HCC组织中AURKA和AURKB的mRNA表达、HCC分期和OS与对照组织有显著差异，但AURKA和AURKB在启动子甲基化水平以及与免疫抑制剂的关系上存在显著不一致。对于AURKC，上述各项均无显著差异。此外，在用Cytoscape软件中的分子复合物检测（MCODE）插件构建的PPI网络中，鉴定出了一个包括AURKA、AURKB和AURKC的枢纽模块。我们的结果表明，AURKB可能是HCC预后的一个潜在生物标志物。
