Garman R D, Doherty P J, Raulet D H
Cell. 1986 Jun 6;45(5):733-42. doi: 10.1016/0092-8674(86)90787-7.
Although the T cell gamma genes are similar in many respects to T cell receptor alpha and beta genes, earlier studies suggested that only a single gamma variable (V gamma) gene is expressed in mature T cells. We report the isolation and characterization of three new rearranged V gamma genes from murine fetal thymocytes. Although each of the new V gamma gene rearrangements is present in fetal thymocytes, two of them are undetectable in mature T cells. The levels of mRNA corresponding to each type of V gamma gene rearrangement in mature T cells are dramatically diminished compared with those in fetal thymocytes, although the abundance of two of the rearranged genes is increased in mature T cells. Our results demonstrate that there is significant expressed variability of gamma genes in immature T cells. Furthermore, the dynamics of gamma gene rearrangement and expression support the idea that gamma genes function in immature T cells.
尽管T细胞γ基因在许多方面与T细胞受体α和β基因相似，但早期研究表明，成熟T细胞中仅表达单个γ可变（Vγ）基因。我们报告了从小鼠胎儿胸腺细胞中分离和鉴定出三个新的重排Vγ基因。虽然每个新的Vγ基因重排在胎儿胸腺细胞中都存在，但其中两个在成熟T细胞中无法检测到。与胎儿胸腺细胞相比，成熟T细胞中对应于每种Vγ基因重排的mRNA水平显著降低，尽管其中两个重排基因的丰度在成熟T细胞中有所增加。我们的结果表明，未成熟T细胞中γ基因存在显著的表达变异性。此外，γ基因重排和表达的动态变化支持了γ基因在未成熟T细胞中发挥作用的观点。
