Department of Endocrinology, Genetics and Metabolism, Children's Hospital of Soochow University, Suzhou, China.
Department of Thyroid and Breast Surgery, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, China.
Front Endocrinol (Lausanne). 2021 Nov 16;12:752496. doi: 10.3389/fendo.2021.752496. eCollection 2021.
Graves' disease (GD) related hyperthyroidism (HT) has profound effects on metabolic activity and metabolism of macromolecules affecting energy homeostasis. In this study, we aimed to get a comprehensive understanding of the metabolic changes and their clinical relevance in GD children.
We investigated serum substances from 30 newly diagnosed GD children and 30 age- and gender-matched healthy controls. We explored the metabolomics using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS) analysis, and then analyzed the metabolomic data multivariate statistical analysis.
By untargeted metabolomic analysis, a total of 730 metabolites were identified in all participants, among which 48 differential metabolites between GD and control groups were filtered out, including amino acids, dipeptides, lipids, purines, etc. Among these metabolites, 33 were detected with higher levels, while 15 with lower levels in GD group compared to controls. Pathway analysis showed that HT had a significant impact on aminoacyl-transfer ribonucleic acid (tRNA) biosynthesis, several amino acids metabolism, purine metabolism, and pyrimidine metabolism.
In this study, untargeted metabolomics analysis, significant variations of serum metabolomic patterns were detected in GD children.
格雷夫斯病（GD）相关的甲状腺功能亢进症（HT）对代谢活性和影响能量平衡的大分子代谢有深远影响。本研究旨在全面了解 GD 儿童的代谢变化及其临床相关性。
我们检测了 30 名新诊断的 GD 儿童和 30 名年龄和性别匹配的健康对照者的血清物质。我们使用超高效液相色谱-四极杆飞行时间质谱联用（UHPLC-QTOF/MS）分析进行代谢组学研究，然后对代谢组学数据进行多元统计分析。
通过非靶向代谢组学分析，在所有参与者中共鉴定出 730 种代谢物，其中 48 种 GD 组和对照组之间的差异代谢物被筛选出来，包括氨基酸、二肽、脂类、嘌呤等。在这些代谢物中，33 种在 GD 组中的水平较高，而 15 种则较低。通路分析表明，HT 对氨酰-tRNA 生物合成、几种氨基酸代谢、嘌呤代谢和嘧啶代谢有显著影响。
在这项研究中，通过非靶向代谢组学分析，检测到 GD 儿童的血清代谢组学图谱存在显著变化。
