de Weger R A, van Loveren H, de Groot J W, Rangarajan R, den Otter W
Immunobiology. 1986 Apr;171(3):170-9. doi: 10.1016/s0171-2985(86)80002-x.
The induction of specific macrophage cytotoxicity by allo-sensitized T cells in vitro is shown to be independent of the presence of lipopolysaccharide (LPS). This contrasts with the induction of macrophage cytotoxicity by Macrophage Activating Factor (MAF). The specific macrophage cytotoxicity could be induced in LPS-free medium (less than l ng/ml). Addition of LPS to the macrophages did not increase the cytotoxicity. Addition of LPS-binding polymyxin B to the macrophages before and during the induction of cytotoxicity did not reduce the specific macrophage cytotoxicity. Macrophages obtained from the LPS-unresponsive mouse strain C3H/HeJ were rendered cytotoxic by the allo-sensitized lymphocytes to the same extent as the macrophages from LPS-responsive C3HeB/Fe and C57BL mice. This indicates that the induction of macrophage cytotoxicity by MAF is different from the induction of specific macrophage cytotoxicity by Specific Macrophage Arming Factor (SMAF).
体外同种致敏T细胞诱导特异性巨噬细胞细胞毒性被证明与脂多糖（LPS）的存在无关。这与巨噬细胞激活因子（MAF）诱导巨噬细胞细胞毒性形成对比。特异性巨噬细胞细胞毒性可在无LPS培养基（低于1 ng/ml）中诱导产生。向巨噬细胞中添加LPS并不会增加细胞毒性。在细胞毒性诱导之前和期间向巨噬细胞中添加LPS结合多粘菌素B并不会降低特异性巨噬细胞细胞毒性。从对LPS无反应的小鼠品系C3H/HeJ获得的巨噬细胞被同种致敏淋巴细胞诱导产生细胞毒性的程度与来自对LPS有反应的C3HeB/Fe和C57BL小鼠的巨噬细胞相同。这表明MAF诱导巨噬细胞细胞毒性与特异性巨噬细胞武装因子（SMAF）诱导特异性巨噬细胞细胞毒性不同。
