Scadding G K, Calder L, Vincent A, Prior C, Wray D, Newsom-Davis J
Immunology. 1986 May;58(1):151-5.
Acetylcholine receptor (AChR)-bearing membranes from the BC3H-1 cell lines were injected, without adjuvant, either intrathymically (i.t.) followed by intraperitoneal (i.p.) booster doses, or i.p. alone, into (C57BL X BALB/c)F1 mice. Over 75% of the animals developed serum anti-AChRs which reacted with the cell-line AChR and with normal mouse endplate AChR. The titres were within the lower range of those of myasthenia gravis patients, and some mice showed reduced miniature endplate potential (m.e.p.p) amplitudes. these results indicate that loss of tolerance to acetylcholine receptors can result from immunization against syngeneic AChR without adjuvant. This approach may provide a useful model for studying mechanisms of autoimmunity against acetylcholine receptor.
将来自BC3H-1细胞系的携带乙酰胆碱受体（AChR）的膜，不添加佐剂，通过胸腺内注射（i.t.），随后腹腔内（i.p.）给予加强剂量，或者仅腹腔内注射，注入（C57BL×BALB/c）F1小鼠体内。超过75%的动物产生了与细胞系AChR以及正常小鼠终板AChR发生反应的血清抗AChR。其滴度处于重症肌无力患者滴度范围的下限，并且一些小鼠表现出微小终板电位（m.e.p.p）幅度降低。这些结果表明，针对同基因AChR进行无佐剂免疫可导致对乙酰胆碱受体的耐受性丧失。这种方法可能为研究针对乙酰胆碱受体的自身免疫机制提供一个有用的模型。
