Xiong Xiao, Kelkar Yogeshwar D, Geden Chris J, Zhang Chao, Wang Yidong, Jongepier Evelien, Martinson Ellen O, Verhulst Eveline C, Gadau Jürgen, Werren John H, Wang Xu
Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States.
School of Life Sciences and Technology, Tongji University, Shanghai, China.
Front Genet. 2021 Nov 12;12:748135. doi: 10.3389/fgene.2021.748135. eCollection 2021.
The parasitoid wasp (Hymenoptera: Pteromalidae) is a gregarious species that has received extensive attention for its potential in biological pest control against house fly, stable fly, and other filth flies. It has a high reproductive capacity and can be reared easily. However, genome assembly is not available for or any other species in this genus. Previously, we assembled a complete circular mitochondrial genome with a length of 24,717 bp. Here, we assembled and annotated a high-quality nuclear genome of , using a combination of long-read (104× genome coverage) and short-read (326× genome coverage) sequencing technologies. The assembled genome size is 314 Mbp in 226 contigs, with a 97.9% BUSCO completeness score and a contig N50 of 4.67 Mb, suggesting excellent continuity of this assembly. Our assembly builds the foundation for comparative and evolutionary genomic analysis in the genus of and possible future biocontrol applications.
寄生蜂（膜翅目：金小蜂科）是一种群居物种，因其在生物防治家蝇、厩螫蝇和其他腐食性蝇类方面的潜力而受到广泛关注。它具有很高的繁殖能力，并且易于饲养。然而，该属的 或任何其他物种都没有可用的基因组组装。此前，我们组装了一个长度为24,717 bp的完整环状线粒体基因组。在此，我们使用长读长（104倍基因组覆盖）和短读长（326倍基因组覆盖）测序技术相结合的方法，组装并注释了 的高质量核基因组。组装后的基因组大小为314 Mbp，分布在226个重叠群中，BUSCO完整性得分97.9%，重叠群N50为4.67 Mb，表明该组装具有出色的连续性。我们的组装为 属的比较和进化基因组分析以及未来可能的生物防治应用奠定了基础。
