Zhang Xiaofeng, Luan Peixian, Cao Dingchen, Hu Guo
National and Local United Engineering Laboratory for Freshwater Fish Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, China.
Front Genet. 2021 Nov 12;12:778487. doi: 10.3389/fgene.2021.778487. eCollection 2021.
Feed conversion efficiency (FCE) is an economically crucial trait in fish, however, little progress has been made in genetics and genomics for this trait because phenotypes of the trait are difficult to measure. In this study, we constructed a high-density and high-resolution genetic linkage map with 28,416 SNP markers for common carp () based on high throughput genotyping with the carp 250K single nucleotide polymorphism (SNP) array in a full-sib F family of mirror carp () consisting of 141 progenies. The linkage map contained 11,983 distinct loci and spanned 3,590.09 cM with an average locus interval of 0.33 cM. A total of 17 QTL for the FCE trait were detected on four LGs (LG9, LG20, LG28, and LG32), explaining 8.9-15.9% of the phenotypic variations. One major cluster containing eight QTL (qFCE1-28, qFCE2-28, qFCE3-28, qFCE4-28, qFCE5-28, qFCE6-28, qFCE7-28, and qFCE8-28) was detected on LG28. Two clusters consisting of four QTL (qFCE1-32, qFCE2-32, qFCE3-32, and qFCE4-32) and three QTL (qFCE1-20, qFCE2-20, and qFCE3-20) were detected on LG32 and LG20, respectively. Nine candidate genes (, , , , , , AGO1, and ) underlying the feed efficiency trait were also identified, the biological functions of which may be involved in lipid metabolism, carbohydrate metabolism, energy deposition, fat accumulation, digestion, growth regulation, and cell proliferation and differentiation according to GO (Gene Ontology). As an important tool, high-density and high-resolution genetic linkage maps play a crucial role in the QTL fine mapping of economically important traits. Our novel findings provided new insights that elucidate the genetic basis and molecular mechanism of feed efficiency and the subsequent marker-assisted selection breeding in common carp.
饲料转化效率（FCE）是鱼类的一个经济上至关重要的性状，然而，由于该性状的表型难以测量，在其遗传学和基因组学方面进展甚微。在本研究中，我们基于鲤鱼250K单核苷酸多态性（SNP）阵列的高通量基因分型，在一个由141个后代组成的镜鲤全同胞F家系中，构建了一个含有28416个SNP标记的高密度、高分辨率的鲤鱼遗传连锁图谱。该连锁图谱包含11983个不同的位点，跨度为3590.09厘摩，平均位点间隔为0.33厘摩。在四个连锁群（LG9、LG20、LG28和LG32）上共检测到17个与饲料转化效率性状相关的QTL，解释了8.9%-15.9%的表型变异。在LG28上检测到一个包含八个QTL（qFCE1-28、qFCE2-28、qFCE3-28、qFCE4-28、qFCE5-28、qFCE6-28、qFCE7-28和qFCE8-28）的主要聚类。在LG32和LG20上分别检测到由四个QTL（qFCE1-32、qFCE2-32、qFCE3-32和qFCE4-32）和三个QTL（qFCE1-20、qFCE2-20和qFCE3-20）组成的两个聚类。还鉴定出九个与饲料效率性状相关的候选基因（、、、、、、AGO1、和），根据基因本体论（GO），其生物学功能可能涉及脂质代谢、碳水化合物代谢、能量沉积、脂肪积累、消化、生长调节以及细胞增殖和分化。作为一种重要工具，高密度、高分辨率的遗传连锁图谱在经济重要性状的QTL精细定位中起着关键作用。我们的新发现为阐明鲤鱼饲料效率的遗传基础和分子机制以及后续的标记辅助选择育种提供了新的见解。
