He Wei, Ni Weijie, Zhao Junning
Department of Gastroenterology, Geriatric Hospital of Nanjing Medical University, Nanjing 210024, China.
School of Medicine, Southeast University, Nanjing 210009, China.
Gastroenterol Res Pract. 2021 Nov 25;2021:2683465. doi: 10.1155/2021/2683465. eCollection 2021.
The involvement of gut microbiota composition in ulcerative colitis is strongly supported by previous research. Growing evidence suggests that probiotic therapy protects against inflammatory bowel disease in animal models and patients. However, as a probiotic, the role of in UC remains unclear. Nevertheless, the potential mechanism of the protective effect of remains unknown. In this study, a dextran sulphate sodium-induced (DSS-induced) colitis model was used to detect the underlying mechanism of in maintaining gut homeostasis. ELISA was performed to detect the levels of cytokines (TNF-, IL-1, IL-6, and IL-10). Furthermore, 454 pyrosequencing was used to investigate the microbiota composition in fecal samples. The results illustrate that administration could prevent DSS-induced gut inflammation and intestinal flora imbalance. At the same time, the damage to intestinal mucosal barrier and tight junctions was partially repaired. These results demonstrate the preventive effect of in DSS-induced intestinal injury. The present study provides new insights into the medicinal value of for UC.
先前的研究有力支持了肠道微生物群组成与溃疡性结肠炎的关联。越来越多的证据表明，益生菌疗法在动物模型和患者中可预防炎症性肠病。然而，作为一种益生菌，其在溃疡性结肠炎中的作用仍不清楚。尽管如此，其保护作用的潜在机制仍然未知。在本研究中，使用葡聚糖硫酸钠诱导（DSS诱导）的结肠炎模型来检测其在维持肠道稳态中的潜在机制。进行酶联免疫吸附测定（ELISA）以检测细胞因子（肿瘤坏死因子-α、白细胞介素-1、白细胞介素-6和白细胞介素-10）的水平。此外，使用454焦磷酸测序来研究粪便样本中的微生物群组成。结果表明，给予该物质可预防DSS诱导的肠道炎症和肠道菌群失衡。同时，肠道黏膜屏障和紧密连接的损伤得到部分修复。这些结果证明了该物质对DSS诱导的肠道损伤具有预防作用。本研究为该物质对溃疡性结肠炎的药用价值提供了新的见解。
