Khair Abul, Awal Mohammed Abdul, Hoque Mohammed Nazmul, Talukder Anup Kumar, Das Ziban Chandra, Rao Damanna Ramkishan, Shamsuddin Mohammed
Quality Control Laboratory, Department of Livestock Services, Savar, Dhaka, Bangladesh.
Department of Pharmacology, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Anim Reprod. 2021 Nov 19;18(3):e20210035. doi: 10.1590/1984-3143-AR2021-0035. eCollection 2021.
Spirulina (), has numerous health benefits including antioxidant, immunomodulatory, and anti-inflammatory activities, works against heavy metal toxicity, and is often used as a food supplement in human, animals, birds and fishes. This study aimed to evaluate the protective ability of the dietary spirulina against the toxic effects of inorganic arsenic (iAs) on male reproductive parameters in rats. Seventy-two mature Long-Evans male rats, dividing into six groups (T0, T1, T2, T3, T4 and T5) (12 rats/group) were included in this study. The T3, T4 and T5 group rats were treated with three consecutive doses (1.0 g, 1.5 g and 2.0 g/kg feed) of spirulina in feed along with 3.0 mg NaAsO/kg body weight (BW) in drinking water (DW) daily for 90 days. Each rat of group T1 received NaAsO (3.0 mg/kg BW) in DW, and those of T2 group were fed with spirulina (2.0 g/kg feed) daily for 90 days. The rats of group T0 served as the control with normal feed and water. Total arsenic (tAs) contents, reproductive parameters (testicular weight, sperm motility and morphology), and histological changes in the testicles were evaluated in these rats. Arsenic dosing significantly (p=0.003, Kruskal-Wallis test) increased the tAs contents in the testicles, decreased testes weight, sperm morphology and motility compared to the controls. The effect of arsenic dosing was also evidenced by the histological changes like decreased germinal layers in the seminiferous tubules of the treated rats. Moreover, dietary spirulina (2.0 g/kg feed) supplementation significantly (p=0.011, Kruskal-Wallis test) lowered tAs contents in testicles and increases testes weights, sperm motility and morphology. Therefore, spirulina can be used as an effective dietary supplement to ameliorate the adverse effects of arsenic induced reproductive toxicities. However, further study is required to elucidate the underlying molecular mechanisms of reduction of arsenic induced reproductive toxicity by spirulina.
螺旋藻具有多种健康益处，包括抗氧化、免疫调节和抗炎活性，可对抗重金属毒性，常用于人类、动物、鸟类和鱼类的食品补充剂。本研究旨在评估日粮螺旋藻对无机砷（iAs）对大鼠雄性生殖参数毒性作用的保护能力。本研究纳入了72只成熟的Long-Evans雄性大鼠，分为六组（T0、T1、T2、T3、T4和T5）（每组12只大鼠）。T3、T4和T5组大鼠在饲料中连续接受三剂（1.0 g、1.5 g和2.0 g/kg饲料）螺旋藻，同时每天在饮用水（DW）中给予3.0 mg NaAsO/kg体重（BW），持续90天。T1组的每只大鼠在DW中接受NaAsO（3.0 mg/kg BW），T2组的大鼠每天喂食螺旋藻（2.0 g/kg饲料），持续90天。T0组的大鼠作为正常饲料和水的对照组。评估了这些大鼠的总砷（tAs）含量、生殖参数（睾丸重量、精子活力和形态）以及睾丸的组织学变化。与对照组相比，砷给药显著（p=0.003，Kruskal-Wallis检验）增加了睾丸中的tAs含量，降低了睾丸重量、精子形态和活力。砷给药的影响还通过组织学变化得到证明，如处理大鼠的生精小管中生发层减少。此外，日粮补充螺旋藻（2.0 g/kg饲料）显著（p=0.011，Kruskal-Wallis检验）降低了睾丸中的tAs含量，并增加了睾丸重量、精子活力和形态。因此，螺旋藻可作为一种有效的日粮补充剂，以改善砷诱导的生殖毒性的不良影响。然而，需要进一步研究以阐明螺旋藻降低砷诱导的生殖毒性的潜在分子机制。
