雄性大鼠的生育能力：砷化合物不良影响的剖析。

Fertility in male rats: Disentangling adverse effects of arsenic compounds.

机构信息

Department of General Biology, Federal University of Viçosa (UFV), Viçosa, Minas Gerais, 36570-900, Brazil.

Department of Veterinary Clinic and Surgery, Veterinary School, Federal University of Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, 31270-901, Brazil.

出版信息

Reprod Toxicol. 2018 Jun;78:130-140. doi: 10.1016/j.reprotox.2018.04.015. Epub 2018 Apr 24.

DOI:10.1016/j.reprotox.2018.04.015

PMID:29702248

Abstract

Arsenic impairs male reproductive functions. However, it is not clear whether different arsenic compounds similarly affect fertility. In this study, we compared the impact of sodium arsenite and arsenate on sperm quality and fertility. After 56 d exposure, male Wistar rats were mated and pregnant females were evaluated by fertility indexes. Clearly, exposure to 10 mg/L arsenite reduced daily sperm production via HO overproduction and germ cells loss. Animals from this group also showed a decrease in epididymal sperm counts and percentage of sperm with intact membranes. Moreover, they presented low fertility potential and high preimplantation loss. In contrast, 10 mg/L arsenate caused oxidative stress in testis, mineral imbalance in epididymis, and sperm membranes damage, with no effects on fertility. Both arsenic compounds at 0.01 mg/L altered reproductive parameters. We concluded that arsenite is more harmful than arsenate to sperm quality and male fertility, with negative influences in early pregnancy.

摘要

砷会损害男性生殖功能。然而，不同的砷化合物是否会对生育能力产生相似的影响尚不清楚。在这项研究中，我们比较了亚砷酸钠和砷酸对精子质量和生育能力的影响。经过 56 天的暴露，雄性 Wistar 大鼠进行交配，通过生育指数评估怀孕雌性的情况。显然，暴露于 10mg/L 的亚砷酸钠通过 HO 过量产生和生殖细胞损失减少每日精子产量。来自该组的动物还表现出附睾精子计数和完整膜精子百分比的减少。此外，它们的生育能力潜力低，着床前损失高。相比之下，10mg/L 的砷酸在睾丸中引起氧化应激，在附睾中引起矿物质失衡和精子膜损伤，但对生育能力没有影响。两种砷化合物在 0.01mg/L 时均改变了生殖参数。我们得出结论，亚砷酸钠对精子质量和男性生育能力的危害大于砷酸，对早期妊娠有负面影响。