Gu Sujie, Peng Zesheng, Wu Yuxi, Wang Yihao, Lei Deqiang, Jiang Xiaobing, Zhao Hongyang, Fu Peng
Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Department of Neurosurgery General Hospital of The Yangtze River Shipping, Wuhan, China.
Front Oncol. 2021 Nov 16;11:752694. doi: 10.3389/fonc.2021.752694. eCollection 2021.
Glioma is the most common malignancy of the central nervous system. Although advances in surgical resection, adjuvant radiotherapy, and chemotherapy have been achieved in the last decades, the prognosis of gliomas is still dismal. COL5A1 is one of the collagen members with minor content but prominent functions. The present study examined the biological functions, prognostic value, and gene-associated tumor-infiltrating immune cells of COL5A1 through experiments and bioinformatics analysis. We found that the overexpression of COL5A1 was positively correlated with the increasing tumor malignancies and indicated poor prognosis in gliomas. Moreover, downregulation of COL5A1 could inhibit proliferation and migration of glioma cells and enhance their temozolomide sensitivities . Further bioinformatic analysis revealed that COL5A1 and its co-expressed genes participated in a number of pathways and biological processes involved in glioma progression. Finally, we evaluated the tumor-infiltrating immune cells of gliomas depending on COL5A1 and found that the percentages of the dendritic cells, which were known as the central mediator of tumor microenvironment in gliomas, were positively associated with the expression levels of COL5A1. Taken together, COL5A1 is an important biomarker and potential therapeutic target of gliomas.
胶质瘤是中枢神经系统最常见的恶性肿瘤。尽管在过去几十年里，手术切除、辅助放疗和化疗都取得了进展，但胶质瘤的预后仍然很差。COL5A1是胶原蛋白成员之一，含量较少但功能显著。本研究通过实验和生物信息学分析，研究了COL5A1的生物学功能、预后价值以及与基因相关的肿瘤浸润免疫细胞。我们发现，COL5A1的过表达与肿瘤恶性程度的增加呈正相关，提示胶质瘤预后不良。此外，COL5A1的下调可抑制胶质瘤细胞的增殖和迁移，并增强其对替莫唑胺的敏感性。进一步的生物信息学分析表明，COL5A1及其共表达基因参与了许多与胶质瘤进展相关的信号通路和生物学过程。最后，我们评估了依赖COL5A1的胶质瘤肿瘤浸润免疫细胞，发现作为胶质瘤肿瘤微环境中心介质的树突状细胞百分比与COL5A1的表达水平呈正相关。综上所述，COL5A1是胶质瘤的一个重要生物标志物和潜在治疗靶点。
