Domian Natalia, Młynarczyk Grzegorz, Kasacka Irena
Department of Histology and Cytophysiology, Medical University of Białystok, Białystok, Poland.
Department of Urology, Medical University of Białystok, Białystok, Poland.
Front Oncol. 2021 Nov 11;11:765640. doi: 10.3389/fonc.2021.765640. eCollection 2021.
The aim of the study was to present a case of penile squamous cell carcinoma and immunohistochemical identification and evaluation of E-cadherin and β-catenin expression.
We are presenting a 70-year old man with a variant of penile squamous cell carcinoma with mixed warty and basaloid features. After diagnosis, the patient underwent partial penectomy. Samples taken from the material after surgery were subjected to basic histological staining and immunohistochemical identification of E-cadherin and β-catenin. A Real-time PCR study was conducted to investigate the expression of E-cadherin and β-catenin.
Routine histopathological examinations revealed the characteristic features of warty-basaloid squamous cell carcinoma. In the case studied, a positive immunohistochemical reaction was observed for E-cadherin and β-catenin. QRT-PCR analysis showed a statistically significant decrease in E-cadherin expression in tumor samples compared to healthy tissue. In contrast, expression of the gene encoding β-catenin was slightly higher in tumor samples compared to normal tissue.
The reduced level of the complex of adhesive elements, E-cadherin-β-catenin, disturbs cell differentiation, promotes a more invasive phenotype-stromal infiltration and the formation of distant metastases. In the described case of the penile tumor, a decrease in E-cadherin expression was noted, which could be related to the occurrence of neoplastic infiltration of the spongy body space. In summary, E-cadherin and β-catenin expression and the immunoreactivity of these proteins are expressed at different levels in tumor cells and in penile interstitial cells. Regulation of expression during various physiological and pathophysiological processes indicates a potentially important role of E-cadherin and β-catenin in cell proliferation and adhesion.
本研究旨在报告一例阴茎鳞状细胞癌病例，并对E-钙黏蛋白和β-连环蛋白进行免疫组化鉴定及表达评估。
我们报告了一名70岁男性，患有具有疣状和基底样混合特征的阴茎鳞状细胞癌变异型。诊断后，患者接受了阴茎部分切除术。术后从标本中取材进行基本组织学染色以及E-钙黏蛋白和β-连环蛋白的免疫组化鉴定。进行实时荧光定量聚合酶链反应（Real-time PCR）研究以检测E-钙黏蛋白和β-连环蛋白的表达。
常规组织病理学检查显示疣状-基底样鳞状细胞癌的特征性表现。在所研究的病例中，观察到E-钙黏蛋白和β-连环蛋白免疫组化反应呈阳性。实时荧光定量聚合酶链反应分析显示，与健康组织相比，肿瘤样本中E-钙黏蛋白表达有统计学意义的降低。相比之下，与正常组织相比，肿瘤样本中编码β-连环蛋白的基因表达略高。
黏附分子复合物E-钙黏蛋白-β-连环蛋白水平降低会干扰细胞分化，促进更具侵袭性的表型——间质浸润和远处转移的形成。在所描述的阴茎肿瘤病例中，观察到E-钙黏蛋白表达降低，这可能与海绵体间隙肿瘤浸润的发生有关。总之，E-钙黏蛋白和β-连环蛋白的表达及其蛋白免疫反应性在肿瘤细胞和阴茎间质细胞中表达水平不同。在各种生理和病理生理过程中表达的调控表明E-钙黏蛋白和β-连环蛋白在细胞增殖和黏附中可能具有重要作用。
