Hu Jiaqing, Li Wenqiang, Qiao Xibo, Li Wenjie, Xie Kerui, Wang Yanyan, Huang Bing, Zhao Qiaoya, Liu Lei, Fan Xinzhong
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, China.
Shandong New Hexin Technology Co. Ltd., Taian, China.
Front Vet Sci. 2021 Nov 17;8:746638. doi: 10.3389/fvets.2021.746638. eCollection 2021.
is the pathogenic agent for a variety of severe diseases in livestock, including rabbits. MicroRNAs (miRNAs) participate in the immune response to the pathogen. Distinct miRNA expression patterns were explored in rabbit lung by small-RNA deep sequencing to assess dysregulated miRNAs during infection. Totally, 571 miRNAs were screened, of which, 62 were novel, and 32 exhibited differential expression (DE). Of the 32 known DE-miRNAs, 13 and 15 occurred at 1 day and 3 days post-infection (dpi); and ocu-miR-107-3p and ocu-miR-29b-5p were shared between the two time points. Moreover, 7,345 non-redundant target genes were predicted for the 32 DE-miRNAs. Putative target genes were enriched in diverse GO and KEGG pathways and might be crucial for disease resistance. Interestingly, upregulation of ocu-miR-29-5p suppresses propagation and downregulates expression of epithelial membrane protein-2 (EMP2) and T-box 4 (TBX4) genes by binding to their 3' untranslated region in RK13 cells. Thus, ocu-miR-29-5p may indirectly inhibit invasion by modulating genes related to the host immune response, such as EMP2 and TBX4.
是包括兔子在内的家畜多种严重疾病的病原体。微小RNA（miRNA）参与对该病原体的免疫反应。通过小RNA深度测序探索了兔肺中不同的miRNA表达模式，以评估感染期间失调的miRNA。总共筛选出571个miRNA，其中62个是新的，32个表现出差异表达（DE）。在32个已知的DE-miRNA中，13个和15个分别在感染后1天和3天出现；ocu-miR-107-3p和ocu-miR-29b-5p在两个时间点都有出现。此外，为32个DE-miRNA预测了7345个非冗余靶基因。推定的靶基因在不同的GO和KEGG途径中富集，可能对疾病抗性至关重要。有趣的是，ocu-miR-29-5p的上调通过在RK13细胞中与其3'非翻译区结合来抑制增殖并下调上皮膜蛋白2（EMP2）和T-box 4（TBX4）基因的表达。因此，ocu-miR-29-5p可能通过调节与宿主免疫反应相关的基因（如EMP2和TBX4）间接抑制侵袭。
