Hu Jiaqing, Li Wenqiang, Huang Bing, Zhao Qiaoya, Fan Xinzhong
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, China.
Shandong Provincial Key Laboratory of Poultry Disease Diagnose and Immune, Institute of Poultry, Shandong Academy of Agricultural Sciences, Jinan, China.
Front Vet Sci. 2021 May 5;8:591273. doi: 10.3389/fvets.2021.591273. eCollection 2021.
Infection with () causes severe epidemic diseases in rabbits and is responsible for the pronounced economic losses in the livestock industry. Long non-coding RNAs (lncRNAs) have been proven to exert vital functions in regulating the host immune responses to bacterial attacks. However, little is known about how lncRNAs participate in the rabbit's immune response against infection in the lungs. LncRNA and mRNA expression profiles were analyzed by transcriptomics and bioinformatics during infection. A total of 336 lncRNAs and 7,014 mRNAs were differentially regulated at 1 day and 3 days post infection (dpi). Nearly 80% of the differentially expressed lncRNAs exhibited an increased expression at 3 dpi suggesting that the genes are responsible for regulation. Moreover, GO and KEGG enriched analysis indicated that the immune-related pathways including pattern recognition receptors (PRRs), cytokines, and chemokines were significantly enriched at 3 dpi. These results indicate that the dysregulated immune-related genes may play crucial roles in defending against attacks. Overall, these results advance our cognition of the role of lncRNAs and mRNAs in modulating the rabbit's innate immune response against attacks, which will offer a valuable clue for further studies into exploring -related diseases in human.
感染（）会在兔子身上引发严重的流行病，并给畜牧业造成巨大经济损失。长链非编码RNA（lncRNAs）已被证明在调节宿主对细菌攻击的免疫反应中发挥重要作用。然而，关于lncRNAs如何参与兔子肺部针对感染的免疫反应，我们知之甚少。在感染期间，通过转录组学和生物信息学分析了lncRNA和mRNA的表达谱。在感染后1天和3天，共有336个lncRNAs和7014个mRNAs受到差异调节。近80%的差异表达lncRNAs在感染后3天表达增加，表明这些基因负责调节。此外，GO和KEGG富集分析表明，包括模式识别受体（PRRs）、细胞因子和趋化因子在内的免疫相关途径在感染后3天显著富集。这些结果表明，失调的免疫相关基因可能在抵御攻击中发挥关键作用。总体而言，这些结果推进了我们对lncRNAs和mRNAs在调节兔子针对攻击的固有免疫反应中作用的认识，这将为进一步研究人类相关疾病提供有价值的线索。
