新型犬 STR 系统在彭布罗克威尔士柯基犬和柴犬群体中的法医应用评估。
Forensic Application Evaluation of a Novel Canine STR System in Pembroke Welsh Corgi and Shiba Inu Groups.
机构信息
Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China.
Multi-Omics Innovative Research Center of Forensic Identification, Department of Forensic Genetics, School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China.
出版信息
Biomed Res Int. 2021 Nov 25;2021:5355109. doi: 10.1155/2021/5355109. eCollection 2021.
AIM
To evaluate the forensic application values of 19 autosomal short tandem repeat (STR) loci in canines.
METHODS
The 19 STR loci in two canine groups (Pembroke Welsh Corgis, = 200; Shiba Inus, = 175) were analysed by the capillary electrophoresis platform. The allele frequencies and forensic parameters were calculated, and the genetic relationships between these two canine groups and a previously reported Labrador group were analysed.
RESULTS
These two canine groups conformed to the Hardy-Weinberg equilibrium at all STRs except for locus VGL3438 in the Pembroke Welsh Corgi group, and there was no linkage disequilibrium among pairwise loci at the 19 STRs. All STRs were polymorphic in the Pembroke Welsh Corgi and Shiba Inu groups, of which the locus C38 had the highest polymorphism. And it was found that the genetic relationship between the Pembroke Welsh Corgi and Labrador groups were closer in the three canine groups (Pembroke Welsh Corgi, Shiba Inu and Labrador).
CONCLUSION
The 19 STR loci had high genetic polymorphisms and forensic application values in Pembroke Welsh Corgi and Shiba Inu groups.
目的
评估 19 个常染色体短串联重复（STR）位点在犬类中的法医学应用价值。
方法
采用毛细管电泳平台分析两组犬（彭布罗克威尔士柯基犬，n=200；柴犬，n=175）的 19 个 STR 位点。计算等位基因频率和法医学参数，并分析这两组犬与之前报道的拉布拉多犬组之间的遗传关系。
结果
除彭布罗克威尔士柯基犬组的 VGL3438 位点外，这两组犬在所有 STR 位点均符合 Hardy-Weinberg 平衡，19 个 STR 位点之间不存在连锁不平衡。在彭布罗克威尔士柯基犬和柴犬组中，所有 STR 位点均具有多态性，其中 C38 位点具有最高的多态性。并且发现在这三个犬组中（彭布罗克威尔士柯基犬、柴犬和拉布拉多犬），彭布罗克威尔士柯基犬和拉布拉多犬组之间的遗传关系更为密切。
结论
19 个 STR 位点在彭布罗克威尔士柯基犬和柴犬组中具有较高的遗传多态性和法医学应用价值。
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