Houbo College of Xinjiang Medical University, No. 12 of Shengli Road, Karamay, Xinjiang, China.
Shihezi University School of Medicine, Beier Road, Shihezi, Xinjiang, China.
Biomed Res Int. 2021 Nov 26;2021:7377299. doi: 10.1155/2021/7377299. eCollection 2021.
In this study, we explored the influence of single nucleotide polymorphism (SNP) in the noncoding region of intercellular adhesion molecule 1 (ICAM1) gene on the occurrence and metastasis of primary hepatocellular carcinoma (PHC).
Sanger sequencing was used to analyze the genotypes of rs3093032, rs923366, and rs281437 locus in the 3'untranslated region (UTR) of the gene. The level of plasma ICAM1 was analyzed by enzyme-linked immunosorbent assay (ELISA).
After adjusting for risk factors such as BMI, smoking, drinking, family history of tumors, and hepatitis B virus test results, the CT genotype at rs3093032 of the gene (OR = 0.19, 95% CI: 0.08-0.44, < 0.01), dominance model (OR = 0.23, 95% CI: 0.11-0.48, < 0.01), and T allele (OR = 0.27, 95% CI: 0.14-0.53, < 0.01) were related to the reduced risk of PHC susceptibility. rs923366 locus CT genotype (OR = 0.63, 95% CI: 0.44-0.90, = 0.01), TT genotype (OR = 0.23, 95% CI: 0.10-0.53, < 0.01), dominant model (OR = 0.55, 95% CI: 0.39-0.77, < 0.01), recessive model (OR = 0.28, 95% CI: 0.12-0.62, < 0.01), and T allele (OR = 0.55, 95% CI: 0.42-0.73, < 0.01) were related to a reduction in the risk of PHC susceptibility. rs281437 locus CT genotype (OR = 2.08, 95% CI: 1.40-3.09, < 0.01), TT genotype (OR = 5.20, 95% CI: 2.22-12.17, < 0.01), dominant model (OR = 2.45, 95% CI: 1.69-3.54, < 0.01), recessive model (OR = 4.32, 95% CI: 1.86-10.06, < 0.01), and T allele (OR = 2.46, 95% CI: 1.79-3.38, < 0.01) were significantly related to the increased risk of PHC susceptibility. SNPs at rs3093032, rs923366, and rs281437 of the gene were significantly correlated with TNM stage and tumor metastasis of PHC patients ( < 0.05).
SNPs at rs3093032, rs923366, and rs281437 in the 3'UTR region of the gene are related to the occurrence and metastasis of PHC.
本研究旨在探讨细胞间黏附分子 1（ICAM1）基因非编码区单核苷酸多态性（SNP）对原发性肝细胞癌（PHC）发生和转移的影响。
采用 Sanger 测序法分析基因 3'非翻译区（UTR）rs3093032、rs923366 和 rs281437 位点的基因型。采用酶联免疫吸附试验（ELISA）分析血浆 ICAM1 水平。
调整 BMI、吸烟、饮酒、肿瘤家族史和乙型肝炎病毒检测结果等危险因素后，基因 rs3093032 的 CT 基因型（OR=0.19，95%CI：0.08-0.44， < 0.01）、显性模型（OR=0.23，95%CI：0.11-0.48， < 0.01）和 T 等位基因（OR=0.27，95%CI：0.14-0.53， < 0.01）与 PHC 易感性降低相关。rs923366 位点 CT 基因型（OR=0.63，95%CI：0.44-0.90， = 0.01）、TT 基因型（OR=0.23，95%CI：0.10-0.53， < 0.01）、显性模型（OR=0.55，95%CI：0.39-0.77， < 0.01）、隐性模型（OR=0.28，95%CI：0.12-0.62， < 0.01）和 T 等位基因（OR=0.55，95%CI：0.42-0.73， < 0.01）与 PHC 易感性降低相关。rs281437 位点 CT 基因型（OR=2.08，95%CI：1.40-3.09， < 0.01）、TT 基因型（OR=5.20，95%CI：2.22-12.17， < 0.01）、显性模型（OR=2.45，95%CI：1.69-3.54， < 0.01）、隐性模型（OR=4.32，95%CI：1.86-10.06， < 0.01）和 T 等位基因（OR=2.46，95%CI：1.79-3.38， < 0.01）与 PHC 易感性增加显著相关。基因 3'UTR 区 rs3093032、rs923366 和 rs281437 的 SNP 与 PHC 患者的 TNM 分期和肿瘤转移显著相关（ < 0.05）。
基因 3'UTR 区 rs3093032、rs923366 和 rs281437 的 SNP 与 PHC 的发生和转移有关。
