From the Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Science, Islamic Azad University, Tehran, Iran.
Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.
Pediatr Infect Dis J. 2021 Dec 1;40(12):e459-e461. doi: 10.1097/INF.0000000000003304.
Human cosavirus (HCosV) is a newly recognized virus that seems to be partly related to nonpolio flaccid paralysis and acute gastroenteritis in pediatric patients. However, the relationship between HCosV and diseases in humans is unclear. To assess an investigation for the occurrence of HCosV among pediatric patients involved in meningitis and encephalitis, we implemented a real-time quantitative polymerase chain reaction assay for detection and quantification of HCosV in stool specimens.
In this study, a total of 160 cerebrospinal fluid samples from September 2019 to October 2020 were collected from presenting pediatric patients with meningitis and encephalitis in a Karaj hospital, Iran. After viral RNA extraction, the real-time quantitative polymerase chain reaction was performed to amplify the 5'Un-Translated Region region of the HCosV genome and viral load was analyzed.
Of the 160 samples tested, the HCosV genomic RNA was detected in 2/160 (1.25%) of samples. The minimum viral load of HCosV was 3.5 × 103 copies/mL from 4 years male patient. The maximum viral load was determined to be 2.4 × 105 copies/mL in one sample obtained from 3.5 years female patient.
This is the first documentation of HCosV detection in cerebrospinal fluid samples that better demonstrates relation of HCosV with neurologic diseases including meningitis and encephalitis. Also, these results indicate that HCosV has been circulating among Iranian pediatric patients.
人类杯状病毒（HCosV）是一种新发现的病毒，它似乎与小儿麻痹症非瘫痪型和急性肠胃炎有关。然而，HCosV 与人类疾病的关系尚不清楚。为了评估人类杯状病毒在参与脑膜炎和脑炎的儿科患者中的发生情况，我们采用实时定量聚合酶链反应检测粪便标本中的 HCosV，并对其进行定量检测。
本研究共收集了 2019 年 9 月至 2020 年 10 月伊朗卡拉季医院就诊的患有脑膜炎和脑炎的儿科患者的 160 份脑脊液样本。提取病毒 RNA 后，采用实时定量聚合酶链反应扩增 HCosV 基因组的 5’非翻译区，并对病毒载量进行分析。
在 160 份检测样本中，有 2/160（1.25%）样本检测到 HCosV 基因组 RNA。HCosV 的最低病毒载量为 3.5×103 拷贝/ml，来自一名 4 岁男性患者。从一名 3.5 岁女性患者获得的一个样本中，病毒载量最高，为 2.4×105 拷贝/ml。
这是首次在脑脊液样本中检测到 HCosV，这进一步表明了 HCosV 与包括脑膜炎和脑炎在内的神经系统疾病之间的关系。此外，这些结果表明 HCosV 一直在伊朗儿科患者中传播。
