伊朗急性胃肠炎儿科患者中人类柯萨病毒的高病毒载量检测
High viral load detection of human Cosavirus in Iranian pediatric patients with acute gastroenteritis.
作者信息
Khoshbazan Sadaf, Ivani Zahra, Mousavi Nasab Seyed Dawood, Ahmadi Nayebali, Parhiz Aynaz, Khalesi Bahman, Firouzjani Mohammad Hassan, Ghaderi Mostafa, Barati Maryam, Ehsani Ardakani Mohammad Javad
机构信息
Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.
Department of Animal Viral Vaccine, Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO) Karaj, Iran.
出版信息
Gastroenterol Hepatol Bed Bench. 2021 Fall;14(Suppl1):S82-S86.
AIM
The present study implemented an RT-qPCR assay for the detection and quantification of human cosavirus in stool specimens from pediatric patients involved in acute gastroenteritis.
BACKGROUND
Human cosavirus is a newly recognized virus that seems to be partly related to acute gastroenteritis in pediatric patients. However, the relationship between human cosavirus and diseases in humans is unclear.
METHODS
From January 2018 to December 2019, a total of 160 stool samples were collected from pediatric patients presenting with acute gastroenteritis in a hospital in Karaj, Iran. After viral RNA extraction, RT-qPCR was performed to amplify the 5'UTR region of the human cosavirus genome and viral load was analyzed.
RESULTS
The human cosavirus genomic RNA was detected in 4/160 (2.5%) stool samples tested. The maximum viral load was determined to be 4.6×10 copies/ml in one sample obtained from a 4-year-old patient.
CONCLUSION
The human cosavirus as a new member of the Picornaviridae family was illustrated in fecal samples from pediatric patients with acute gastroenteritis in Iran. This is the first documentation of human cosavirus circulation in Iranian children.
目的
本研究采用逆转录定量聚合酶链反应（RT-qPCR）检测方法，对参与急性胃肠炎的儿科患者粪便标本中的人杯状病毒进行检测和定量分析。
背景
人杯状病毒是一种新发现的病毒，似乎与儿科患者的急性胃肠炎有一定关联。然而，人杯状病毒与人类疾病之间的关系尚不清楚。
方法
2018年1月至2019年12月，从伊朗卡拉季一家医院的急性胃肠炎儿科患者中总共收集了160份粪便样本。提取病毒RNA后，进行RT-qPCR以扩增人杯状病毒基因组的5'非翻译区（5'UTR），并分析病毒载量。
结果
在检测的160份粪便样本中，有4份（2.5%）检测到了人杯状病毒基因组RNA。从一名4岁患者获得的一份样本中，确定最大病毒载量为4.6×10拷贝/毫升。
结论
人杯状病毒作为小RNA病毒科的一个新成员，在伊朗急性胃肠炎儿科患者的粪便样本中得到证实。这是伊朗儿童中人杯状病毒传播的首次记录。
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