钙指示剂Fura-2揭示中枢神经系统有髓轴突中依赖活动的钙瞬变。
Activity-dependent calcium transients in central nervous system myelinated axons revealed by the calcium indicator Fura-2.
作者信息
Lev-Ram V, Grinvald A
机构信息
Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel.
出版信息
Biophys J. 1987 Oct;52(4):571-6. doi: 10.1016/S0006-3495(87)83246-0.
Abstract
Optical measurements from rat optic nerve, loaded with the new Ca2+ indicator Fura-2, provide the first evidence for the presence of activity-dependent fast intracellular [Ca2+] transients in mammalian central nervous system (CNS) myelinated axons. The results suggest that voltage-dependent Ca2+ channels are present in some of the myelinated axons. Optical measurements from axons stained with anterogradely transported voltage-sensitive dye suggest the presence of Ca2+-dependent potassium conductances in these axons. This report also demonstrates that Fura-2 can readily detect changes in [Ca2+] inside cells as a result of electrical activity, and establishes its suitability for measurements of intracellular Ca2+ transients in the millisecond time domain.
摘要
用新型钙离子指示剂Fura-2加载大鼠视神经进行光学测量，首次证明了在哺乳动物中枢神经系统（CNS）有髓轴突中存在活动依赖性快速细胞内[Ca2+]瞬变。结果表明，一些有髓轴突中存在电压依赖性Ca2+通道。用顺行运输的电压敏感染料染色的轴突进行光学测量表明，这些轴突中存在Ca2+依赖性钾电导。本报告还证明，Fura-2能够很容易地检测到电活动引起的细胞内[Ca2+]变化，并确定其适用于在毫秒时域测量细胞内Ca2+瞬变。
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